California Institute of Technology

The United States of America

Sonja Hess

Michael Sweredoski

Annie Moradian

Roxana Eggleston-Rangel

John R Lloyd

Tanya R Yakushi

A method for preparing a methionine containing peptide or protein for analysis by mass spectrometry by incubating the methionine containing peptide or protein with a heterologous methionine sulfoxide reductase A (MsrA), a heterologous methionine sulfoxide reductase B (MsrB), or a heterologous methionine sulfoxide reductase AB (MsrAB) enzyme results in the reduction of an oxidized methionine containing peptide or protein.

Reduction of oxidated methionine peptides for mass spectrometry

A single electrode electrochemical/electrospray ionization source using a corona discharge and a method of analyzing a sample using a corona discharge single electrode electrochemical/electrospray ionization source are provided. In the corona discharge single electrode electrochemical/electrospray ionization technique electrons are removed from the metal tip of the device through gases present in the electrospray ion source resulting in electrochemical ionization of the sample of interest. The resulting odd electron sample cation (positive ion mode) or anion (negative ion mode) can then be analyzed by an appropriate technique, such as, for example, a mass spectrometer.

Single electrode corona discharge electrochemical/electrospray ionization

Growing community of inventors

Arcadia, CA, United States of America

Sonja Hess