California Institute of Technology

Pact Pharma, Inc.

Adoc Ssf, LLC

Gwangju Institute of Science and Technology

Songming Peng

Olivier Dalmas

James R Heath

Alex Franzusoff

Barbara Sennino

Stefanie Mandl-Cashman

Xiaoyan Robert Bao

Alphonsus Hon-Chung Ng

Zheng Pan

Michael Bethune

Boi Bryant Quach

Duo An

Sung Jae Yang

Ji-Chul Hyun

William Chour

Jongchan Choi

Disclosed herein are antigenic peptide-MHC complexes, termed comPACT polypeptides and comPACT polynucleotides, and methods of producing such complexes. Also discloses herein are methods of producing libraries of comPACT polynucleotides and polypeptides, and their exemplary use in capturing cancer neoepitope-reactive T cells with high accuracy. Dual particle detection approaches for detection of neoantigen specific T cells with improved sensitivity and specificity are provided. Signal to noise ratio analysis of isolated T cells for detection of neoantigen-specific T cells with improved T cells is also provided.

Compositions and methods for identification of antigen specific T cells

Compositions and methods for identifying antigen-specific T cells, including determining paired T cell receptor sequences for a specific antigen, are described. Compositions and methods for identifying neoantigen-specific T cells are also described. Microfluidic devices useful for identifying antigen-specific T cells, and methods of using the same, are also described.

Pairing antigen specificity of a T cell with T cell receptor sequences

Disclosed herein are antigenic peptide-MHC molecules, termed comPACTs, and methods of producing such molecules. Also disclosed herein are methods of producing libraries of comPACT polynucleotides and polypeptides, and their exemplary use in capturing cancer neoepitope-reactive T cells.

Peptide-MHC compacts

Disclosed herein are methods and devices for antigen-specific T cell identification or neoantigen identification. Also disclosed herein are devices for separating and isolating antigen-specific T cells or other particles of a certain size from a population of particles of different sizes. Also describe herein are methods and devices for the separation and isolation of barcoded T cells from other nanoparticles containing barcodes for subsequent analysis and further processing of a viable T cell.

Microfluidic devices and methods for purifying rare antigen-specific T cell populations

Compositions and methods for isolating patient-derived antigen-specific T cells include an antigen complex having a polynucleotide barcoded nanoparticle sorting agent complexed with a peptide-loaded streptavidin major histocompatability complex (MHC) tetramer, the barcoding technology allowing for high fidelity screening of a library of the antigen complexes to readily isolate and identify antigen-specific T cells.

Compositions and methods for screening T cells with antigens for specific populations

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San Mateo, CA, United States of America

Songming Peng