Becton Dickinson and Company Limited

Song Shi

Richard L Moore

James A Garrett

John D Mantlo

James G Nadeau

Adrien P Malick

Robert W Rosenstein

Susan M Kircher

William B Brasso

Liping Feng

Vanda White

Richard Ivey

Michael A Brasch

Nicholas Robert Bachur, Jr

Gregory Tice

Donald W Copertino

Paul Ernest Goldenbaum

Thomas M Gentle, Jr

Jon E Salomon

Tuan-Linh Ngoc Nguyen

Michael L Towns

Xiao Mo

Thomas M Gentle

Xiao Li

Kenneth Anthony Kopher

Gary Siuzdak

Elizabeth J Want

Zhouxin Shen

Axel A Yup

William Alfred Pope

Klaus W Berndt

Christopher Austin Battles

Benjamin R Bartfeld

Jamieson W Crawford

Ravi Attri

Robert J Losada

Gregory R Hires

Mei Yang-Woytowitz

Stephen J Lovell

Ben Turng

Julie L Rosales

Xiaofei Chang

Dyan Luper

James Y Zhou

Gretta Campbell

Yongqiang Zhang

Mei Yang

Thomas Gentle

A container for culturing a blood sample. The container has a reservoir that is no larger than about 40 ml in volume with culture media therein varying in amount by volume 0.5 ml to about 20 ml. The container is adapted to receive a blood sample drawn from a patient, wherein the blood volume is about 1 ml to about 20 ml. In some embodiments the ratio of blood volume to culture media volume is about 2:1 to about 1:2 and the volume of blood does not exceed about 10 ml. In some embodiments, the media is lytic media. A method for using the container to culture a blood sample is also contemplated. In such method, the container is inoculated with the blood sample. In certain embodiments, the volume of the blood sample does not exceed 10 mls.

Blood culture system with low media volume

A method for detecting the presence of a microorganism in a fluid sample is disclosed. The method includes providing a fluid specimen within a specimen collection container having a sidewall defining an interior therein. The interior includes a mechanical separator adapted for separating the fluid sample into first and second phases within the specimen collection container and a sensing element capable of detecting the presence of a microorganism disposed therein. The method includes subjecting the specimen collection container to applied rotational force to isolate a concentrated microorganism region. The method also includes detecting by a sensing element the presence or absence of a microorganism within the concentrated microorganism region.

Method and apparatus for microorganism detection

Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.

Identification of microorganisms using MALDI-TOF-MS on-plate extraction

The present invention relates to methods, monitors and systems, useful, for example, for advanced detection of sepsis in a subject.

Advanced detection of sepsis

An array of micro-chambers () with ISFETs () disposed therein for monitoring single cell activity in the microarray to determine the presence or absence of microorganisms in a sample ().

Methods and apparatus for rapid detection of infectious microorganisms

Described herein are methods and reagents for identifying and analyzing at least one microorganism (e.g. bacteria) in a sample and reducing the background signal intensity obtained when analyzing the sample by flow cytometry. The sample is prepared by combining the sample with a background signal-reducing molecule or with a nucleic acid stain covalently linked to a quencher. A portion of the particulate matter in the sample can optionally be removed with a resin prior to staining with a nucleic acid stain.

Sample preparation for flow cytometry

An array of micro-chambers () with individual ion sensitive field effect transistors (ISFETs) () disposed therein for monitoring single cell activity in the microarray to determine the presence or absence of microorganisms in a sample (). In addition to the presence or absence of a single cell, certain further embodiments contemplate monitoring cell behavior. Cell behavior includes the entire range of cell activity as well as cell response to changes in environmental conditions of changes in response due to the addition of sample constituents.

A process for determining one of the presence, absence, or total of microorganisms (e.g. bacteria) in a sample. According to the process, a biological sample containing complex matrices is obtained. The sample is first combined with a resin to adsorb complex matrices from the sample. The resin is removed from the biological sample. The sample so prepared is then analyzed by flow cytometry.

Method for improving analysis of microorganisms in complex matrices

The present invention relates to methods, monitors and systems measuring lysophosphatidylcholine, its derivatives and/or procalcitonin as well as at least one clinical marker (e.g. temperature or respiration rate) and/or at least one biomarker for the early detection of sepsis in a subject.

Methods of the invention include the isolation of intact, viable microorganism(s) from positive blood culture ('PBC') samples for use in downstream analyses such as identification and antimicrobial susceptibility testing ('AST'). The methods involve collecting a portion of the PBC sample, adding a choline-containing solution, lysing the blood cells, isolating the viable microorganism, and performing downstream analysis of the isolated, viable microorganism. The methods can be applied to a variety of gram-positive bacteria, gram-negative bacteria, and/or yeast, and particularly to strains of

Method for direct and rapid identification of microorganisms and antimicrobial susceptibility testing from positive blood cultures

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Reisterstown, MD, United States of America

Song Shi