Cellectis

Chromatin, Inc.

The University of Chicago

Song Luo

Daphne Preuss

Gregory Paul Copenhaver

Amin Hassanzadeh

Shaan Mulchandani

Shimon Modi

Walid Negm

Kevin Keith

James J Solderitsch

Nicholas J Baltes

Shawn R Carlson

Pierluigi Barone

Daniel F Voytas

Feng Zhang

Jennifer M Mach

Thomas Stoddard

Jin Li

Materials and methods for creating plants (e.g., alfalfa lines) with reduced lignin content and composition are provided herein, as are plants, plant parts, and plant cells generated by the methods provided herein.

Alfalfa with reduced lignin composition

Materials and methods are provided for making plants with altered levels of amino acids, particularly by making controlled frameshift mutations in genes that are highly expressed in plant leaves or plant seeds.

Methods for altering amino acid content in plants through frameshift mutations

Materials and methods for genome engineering through transient expression of a targeted nuclease are described herein. For example, the methods described herein can include introducing into a cell a messenger RNA (mRNA) that encodes a nuclease targeted to a selected sequence within the cell, where the stability of the mRNA is modified by the addition of untranslated regions (UTRs).

Modifying messenger RNA stability in plant transformations

The invention is generally related to MCs containing sorghum centromere sequences. In addition, the invention provides for methods of generating plants transformed with these MCs. MCs with novel compositions and structures are used to transform plants cells which are in turn used to generate the plant. Methods for generating the plant include methods for delivering the MC into plant cell to transform the cell, methods for selecting the transformed cell, and methods for isolating plants transformed with the MC.

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The invention is generally related to Sugarcane mini-chromosomes and recombinant chromosomes containing Sugarcane centromere sequences. In addition, the invention provides for methods of generating Sugarcane plants transformed with these Sugarcane mini-chromosomes. Sugarcane mini-chromosomes with novel compositions and structures are used to transform Sugarcane cells which are in turn used to generate Sugarcane plants. Methods for generating Sugarcane plants include methods for delivering the Sugarcane mini-chromosomes into Sugarcane cell to transform the cell, methods for selecting the transformed cell, and methods for isolating Sugarcane plants transformed with the Sugarcane mini-chromosome or recombinant chromosome.

Sugarcane centromere sequences and minichromosomes

The invention provides efficient methods for the isolation of centromeres from potentially any organism. Using the methods of the invention, methylated centromere DNA may be isolated from potentially any centromere in an organism. The technique is amenable to mass screenings employing use of arrays comprising libraries of DNA from a target species.

Use of methylated nucleic acid segments for isolating centromere DNA

The invention provides efficient methods for the isolation of centromeres from potentially any organism. The methods may comprise the steps of: a) preparing a first sample of genomic DNA from a selected species; b) obtaining a plurality of methylated nucleic acid segments from the genomic DNA; and c) screening the methylated nucleic acid segments to identify a centromere nucleic acid sequence.

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Chicago, IL, United States of America

Song Luo