University of Florida Research Foundation, Incorporated

Louisiana State University and Agricultural & Mechanical College

Sonal Sanjeev Tuli

Gregory Scott Schultz

Alfred Samuel Lewin

David Clair Bloom

Zachary L Watson

Donna M Neumann

Daniel J Gibson

Jia Liu

Aspects of the application relate to methods and compositions for delivering therapeutic nucleic acids to neural cells or tissue in a subject. Additional aspects of the application relate to therapeutic nucleic acids, for example therapeutic ribozymes, that are useful for inhibiting viral reactivation in a subject.

Method for delivering RNA to neurons to treat herpes infections

Electromotive delivery of macromolecules can be provided using a delivery device including a first chamber for contacting a macromolecule delivery medium to a surface of a tissue or other anatomy of a patient; and a second chamber for contacting a receiving medium to the patient. A first electrode can be disposed in the first chamber so as to not directly contact the macromolecule delivery medium. A second medium having a buffering agent can be used to keep the first electrode from coming into direct contact with the macromolecule delivery medium. A second electrode can be disposed within the receiving medium in the second chamber such that the second electrode does not directly contact the patient. An electric field can be generated using the first and second electrodes in order to cause the macromolecule delivery medium to move into the tissue of interest from the first chamber of the delivery device.

Method and device for electromotive delivery of macromolecules into tissue

Hammerhead ribozymes that target components critical to HSV replication (ICP4, U20, U30, and U54) were synthesized and shown to efficiently cleave target RNA encoding a portion of these components in in vitro assays. Several cloned cell lines stably expressing these ribozymes were established. HSV-mediated plaque formation was dramatically reduced in cells stably transfected with an anti-HSV ribozyme compared to non-transfected cells.

Herpesvirus ribozymes and vectors

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Gainesville, FL, United States of America

Sonal Sanjeev Tuli