10x Genomics, Inc.

Solongo B Ziraldo

Serge Saxonov

Christopher Hindson

John R Stuelpnagel

Katherine Pfeiffer

Andrew D Price

Tarjei Sigurd Mikkelsen

Geoffrey McDermott

Zachary Bent

Paul Ryvkin

Jessica Michele Terry

Josephine Harada

Elliott Meer

Christopher Joachim O'Keeffe

Phillip Belgrader

Rajiv Bharadwaj

Michael Ybarra Lucero

Tobias Daniel Wheeler

Mohammad Rahimi Lenji

Niranjan Srinivas

Vijay Kumar Sreenivasa Gopalan

Indira Wu

Stephane Claude Boutet

Sarah Taylor

Shea Thompson Lance

The present disclosure relates to methods, compositions and systems for droplet processing. For example, a method can include (a) partitioning a plurality of cells into a plurality of aqueous droplets, wherein an aqueous droplet of the plurality of aqueous droplets comprises a cell of the plurality of cells; and delivering a micellized lysis agent to the cell in the aqueous droplet.

Analysis of nucleic acid sequences

The present disclosure relates to methods, compositions and systems for droplet processing. For example, methods can include (a) providing a first emulsion comprising a first droplet population, wherein droplets of the first droplet population comprise a lysis reagent, and a second emulsion comprising a second droplet population, wherein a droplet of the second droplet population comprises i) a cell or a nucleus, and ii) a plurality of nucleic acid barcode molecules; (b) subjecting the first emulsion and second emulsion to conditions sufficient to transfer the lysis reagent to the second droplet population via micelles comprising the lysis reagent; and (c) lysing the cell or the nucleus within the droplet of the second droplet population with the lysis reagent.

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for 'tagging' analytes for characterization.

Methods and systems for processing polynucleotides

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

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Pleasanton, CA, United States of America

Solongo B Ziraldo