10x Genomics, Inc.

Solongo B Ziraldo

Geoffrey McDermott

Serge Saxonov

Christopher Hindson

John R Stuelpnagel

Katherine Pfeiffer

Andrew D Price

Tarjei Sigurd Mikkelsen

Zachary Bent

Paul Ryvkin

Jessica Michele Terry

Josephine Harada

Elliott Meer

Christopher Joachim O'Keeffe

Shea Thompson Lance

Phillip Belgrader

Rajiv Bharadwaj

Michael Ybarra Lucero

Tobias Daniel Wheeler

Josephine Lee

Francesca Meschi

Mohammad Rahimi Lenji

Vijay Kumar Sreenivasa Gopalan

Niranjan Srinivas

Luz Montesclaros

Indira Wu

Stephane Claude Boutet

Sarah Taylor

Joseph Francis Shuga

Geoffrey Mcdermott

Samuel Marrs

The disclosure provides systems and methods for droplet processing. For example, a method can include providing a first droplet and a second droplet. In some cases, the first droplet may have a first concentration of a reagent and the second droplet may have a second concentration of the reagent. The second droplet may comprise a bead or a biological particle. The method can also include subjecting the first droplet and the second droplet to conditions sufficient to transfer the reagent from the first droplet to the second droplet, thereby decreasing the first concentration in the first droplet and increasing the second concentration in the second droplet.

Systems and methods for transfer of reagents between droplets

The present disclosure relates to methods, compositions and systems for droplet processing. For example, a method can include (a) partitioning a plurality of cells into a plurality of aqueous droplets, wherein an aqueous droplet of the plurality of aqueous droplets comprises a cell of the plurality of cells; and delivering a micellized lysis agent to the cell in the aqueous droplet.

Analysis of nucleic acid sequences

The present disclosure relates to methods, compositions and systems for droplet processing. For example, methods can include (a) providing a first emulsion comprising a first droplet population, wherein droplets of the first droplet population comprise a lysis reagent, and a second emulsion comprising a second droplet population, wherein a droplet of the second droplet population comprises i) a cell or a nucleus, and ii) a plurality of nucleic acid barcode molecules; (b) subjecting the first emulsion and second emulsion to conditions sufficient to transfer the lysis reagent to the second droplet population via micelles comprising the lysis reagent; and (c) lysing the cell or the nucleus within the droplet of the second droplet population with the lysis reagent.

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for 'tagging' analytes for characterization.

Methods and systems for processing polynucleotides

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

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Pleasanton, CA, United States of America

Solongo B Ziraldo