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The United States of America As Represented by the Secretary of

Solomon K Sackitey

Pina M Fratamico

Martin Wiedmann

Primers specific for enterohemorrhagic Escherichia coli (EHEC) 0157:H7 bacteria have been designed which are useful for detecting the bacteria by polymerase chain reaction methods. The primers were derived from DNA sequences contained within a 60-MDa plasmid which is present in most EHEC. The primers may also be used in combination with primers derived from other sequences of significance, the conserved sequences of Shiga-like toxins I and II and the eaeA gene, in a single simultaneous amplification reaction to specifically identify EHEC serotype 0157.

Assay for enterohemorrhagic Escherichia coli 0157:H7 by the polymerase

A game apparatus and an asociated method of play that educates the players about DNA related technologies. The game includes a selector for selecting a nucleotide from a group of nucleotides normally associated with DNA. By randomly selecting nucleotides and recording the selected nucleotides, each player creates a unique DNA sequence. The DNA sequence is used in one of a variety of game motifs to determine the winner of the game.

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Norristown, PA, United States of America

Solomon K Sackitey