Trans Bio-diesel Ltd.

Other

Enzymotec Ltd.

Enzymocore Ltd.

Metallo Therapy Ltd.

Sobhi Basheer

Avidor Shulman

Rassan Zuabi

Orly Farkash

Gai Ben-Dror

Dorit Plat

Ahmad Egbarieh

Maisa Haj

Ramez Masri

Usama Mohsen

Ahmad Hindawi

Emad Masoud

Doaa Shehadeh

Amal Ayoub

Nassim Safadi

Muhammad Kaiyal

Neta Scheinman

Einav Gako-Golan

Elzaphan Hotam

Zohar Goldshmit

Disclosed are various enzymatic processes for the enrichments of oils with omega-3 fatty acids, and specific lipase preparations for use with these processes.

Enzymatic enrichment of n-3 fatty acids in the form of glycerides

Disclosed are an enzymatic batchwise or continuous process for the production of fatty acid alkyl esters for use in the biofuels, food and detergent industries and a system therefor. The process utilizes enzymes immobilized on a hydrophobic resin mixed with a fatty acid source and an alcohol or alcohol donor in the presence of an alkaline or mild alkaline aqueous buffer, or in the presence of water or aqueous solution. The production process for fatty acid alkyl esters is carried out by transesterification or esterification simultaneously or sequentially. The biocatalyst activity is maintained with no significant activity losses in multiple uses and also avoids the accumulation of glycerol and water by-products or other hydrophilic compounds on the biocatalyst.

Enzymatic transesterification/esterification processes employing lipases immobilized on hydrophobic resins in the presence of water solutions

Disclosed are processing systems and processes for carrying out enzymatic batchwise or continuous process for the production of fatty acid alkyl esters for use in the biofuels, food and detergent industries.

Surface-modified heavy metal nanoparticles, including a heavy metal core and a coating layer, the coating layer having at least one ligand, conjugated to polyethylene glycol, the at least one ligand is selected from N-acetyl cysteine, albumin, cysteine, methionine, glutathione, amino thiols, thio-carboxylic acids, ammonia, amines, diamines or any combination thereof. Compositions including surface-modified heavy metal nanoparticles and uses thereof in treatment and diagnosis of various conditions.

Surface-modified heavy metal nanoparticles, compositions and uses thereof

Disclosed are preparations of modified interfacial enzymes, particularly lipases and phospholipases, immobilized on a solid support, wherein the enzyme is surrounded by hydrophobic microenvironment, thereby protected from deactivation and/or aggregation in the presence of hydrophilic agents, substrates and/or reaction products. The enzyme may be protected by being covalently bonded with lipid groups which coat the enzyme, or by being immobilized or embedded in a hydrophobic solid support. Also disclosed are processes for the preparation of the hydrophobically protected enzymes. The enzymes may be efficiently used in the preparation of biodiesel.

Modified-immobilized enzymes of high tolerance to hydrophilic substrates in organic media

Disclosed is an enzymatic process for the preparation of fatty acid alkyl esters, particularly fatty acids methyl esters (biodiesel) in a solvent-free microaqueous system, from a fatty acid source and an alcohol or alcohol donor, employing a robust lipase preparation that comprises at least two lipases separately or jointly immobilized on a suitable support, where one of the lipases has increased affinity to partial glycerides, another is sn-1,3 positional specific, and an optional third lipase has high selectivity towards sn-2 position of the glycerol backbone of the fatty acid source.

Robust multi-enzyme preparation for the synthesis of fatty acid alkyl esters

Disclosed is a process for the preparation of an interfacial enzyme immobilized on an insoluble support, by providing a bi-phase system comprised of an aqueous buffer solution and at least one first organic solvent; mixing said interfacial enzyme with the bi-phase system provided; adding the support to the obtained mixture and mixing; and isolating from the mixture obtained in the last step the interfacial enzyme immobilized on said support. The produced enzyme is locked in its catalytically active confirmation, and thus exhibits improved activity and stability. Also disclosed are uses of the produced enzymes, particularly in the preparation of biodiesel.

Immobilized interfacial enzymes of improved and stabilized activity

A process for the preparation of short-chain alkyl esters of fatty acids, preferably fatty acid methyl esters (biodiesel) in solvent-free system is disclosed. In particular, the process comprises (a) providing a fatty acid source, (b) stepwise adding a short-chain free alcohol, preferably methanol, or any other alcohol donor, to said fatty acid source in the presence of a lipase preparation and (c) allowing the reaction to proceed under suitable conditions, until said fatty acid source triglycerides are converted to fatty acid methyl esters (FAME). The lipase preparation comprises at least two lipases. The two lipases being separately or jointly immobilized on a suitable support and wherein at least one of the lipases has increased affinity for partial glycerides and at least one of the lipases is sn-1,3 positional specific.

A new enzymatic process for preparing 1,2-diacylated phospholipids using an enzyme preparation possessing phospholipase activity towards acylation at the sn-1 and sn-2 sites in a microaqueous reaction system. More particularly, the 1,2-diacyl-phospholipids produced according to the esterification/transesterification process are obtainable in high yield and purity and carry identical desired carboxylic acid, preferably fatty acid, acyl groups at the sn-1 and sn-2 positions. The process involves esterification/transesterification (acylation) of a glycerophospholipid, preferably glycerophosphoryl choline (GPC) with a desired carboxylic acid, preferably fatty acid, or their derivatives in the presence of the above mentioned appropriate enzyme preparation. The process of the invention further relates to a process for the production of 1-acyl-2-lyso-glycerophospholipid, preferably 2-lyso-PC by reacting glycerophospholipid, preferably glycerophosphoryl choline (GPC) with a desired carboxylic acid, preferably fatty acid, or their derivatives in the presence of a sn-1 specific phospholipase (PLAor PLA) and a solvent, in a microaqueous medium.

Process for the production of phospholipids

A lipase preparation is prepared containing a surfactant-coated lipase complex immobilized onto an insoluble matrix. A preferred surfactant is a fatty acid polyol ester such as sorbitan monostearate (SMS). The lipase preparation may be provided in granulated form or in an organic solvent, and can be used in esterifying, inter-esterifying, trans-esterifying and alcoholysing reactions with no added water. The lipase preferably has 1,3-positional specificity with respect to triacylglycerols, and the insoluble matrix may be modified with a fatty acid derivative. A lipase in aqueous medium is contacted with a surfactant to coat the lipase with the surfactant, and the coated lipase is immobilized onto an insoluble matrix to produce the lipase preparation which may be dried such as by freeze drying. Alternatively, the lipase may be first contacted with the insoluble matrix, and thereafter with the surfactant.

Growing community of inventors

Sakhnine, Israel

Sobhi Basheer