Complete Genomics, Inc.

Mgi Tech Co., Ltd.

Callida Genomics, Inc.

Bgi Shenzhen

Nuvelo, Inc.

Other

Hyseq, Inc.

Bgi-shenzhen Co., Limited

Snezana Drmanac

Radoje Drmanac

Matthew J Callow

George Yeung

Brian K Hauser

Chongjun Xu

Handong Li

David B Kita

Wenwei Zhang

Xun Xu

Yuliang Dong

Cory Cooke

Lewis T Williams

Giulia C Kennedy

Michael A Innis

Klaus Giese

Christoph Reinhard

Jaime A Escobedo

Filippo M Randazzo

David Pot

Pablo Dominguez Garcia

Ivan Labat

Yue Zheng

Naibo Yang

George Lamson

Altaf Kassam

Julie Sudduth-Klinger

Birgit Stache-Crain

Zhougang Zhang

Dena Leshkowitz

Guangyang Cai

Veronica Garcia

Lee William Jones

Mark Dickson

Radomir Crkvenjakov

Leon Eckhardt

Scott Gablenz

Lingling Peng

Rongqin Ke

Xi Zhang

Lin Wang

Brian Hauser

Yujun Zhou

Eric Harness

Shaohua Xiang

Fen Liu

Huanhuan Liu

Aaron Hou

Yongwei Zhang

Quanxin Yun

Matthew Callow

Random arrays of single molecules are provided for carrying out large scale analyzes, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer. Preferably, such regions have areas substantially less than 1 μmand have nearest neighbor distances that permit optical resolution of on the order of 10single molecules per cm. Many analytical chemistries can be applied to random arrays of the invention, including sequencing by hybridization chemistries, sequencing by synthesis chemistries, SNP detection chemistries, and the like, to greatly expand the scale and potential applications of such techniques.

DNA array

DNA concatemers on a surface

Reversibly blocked nucleoside analogues and methods of using such nucleoside analogues for sequencing of nucleic acids are provided.

Reversibly blocked nucleoside analogues and their use

The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.

Nucleic acid sequencing using affinity reagents

Methods and compositions for protecting DNA from light-induced damage and other modifications that occur during DNA sequencing using fluorescent dyes are disclosed.

DNA protection agent in DNA imaging buffer

This application discloses methods of producing a DNA strand for sequencing, as well as genetic constructs, libraries, and arrays using DNA strands produced according to these methods. The application also discloses methods of sequencing using the DNA strands, genetic constructs, libraries, and arrays produced. In certain aspects, DNA being sequenced includes a target sequence and at least one adaptor sequence.

DNA sequencing using controlled strand displacement

Provided are a phi29 DNA polymerase mutant with increased thermo stability, a method for preparing the mutant, the use of the mutant, and a method for increasing the stability of the phi29 DNA polymerase.

PHi29 DNA polymerase mutant with increased thermostability

Disclosed in the present disclosure is a recombinant DNA polymerase. The recombinant DNA polymerase is any one selected from: A) a protein, having amino acid modifications at positions 408, 409 and 485, and at least one of amino acid modification(s) at positions 53, 59, 199, 243, 526, 558, 613, 641, 671, 673, 674, 692 and 709 compared to the amino acid sequence of a wild-type KOD DNA polymerase; B) a protein derived from the protein in A), formed by deleting amino acids 1 to 29 from a C-terminus of the protein in A) and keeping the remaining amino acids unchanged; and C) a protein derived from the protein in A) or B), formed by connecting a tag to the N-terminus or C-terminus of the amino acid sequence of the protein in A) or B), wherein the protein in A), B) and C) has DNA polymerase activity.

DNA polymerase variant and application thereof

Provided are a phi29 DNA polymerase and an encoding gene and an application thereof. The phi29 DNA polymerase is C1) or C2): C1) is a protein with DNA polymerase activity obtained by substituting at least one of the 58, 61, 94, 96, 119, and 155amino acid residues in the amino acid sequence of a wild type phi29 DNA polymerase as shown in SEQ ID NO: 2 in the sequence listing; and C2) is a fusion protein obtained by linking a label to the N-terminus and/or C-terminus of the protein represented by C1). A 3'-5&#x2032;exonuclease of the phi29 DNA polymerase has activity lower than that of the wild type phi29 DNA polymerase, and can efficiently and continuously synthesize DNA during amplification and sequencing.

Phi29 DNA polymerase and encoding gene and application thereof

The invention relates to an automated method for high-throughput DNA sequencing from high density DNA arrays by (a) initiating a first sequencing reaction on a first high density DNA array; and imaging said first high density DNA array using a detector, and (b) initiating a first sequencing reaction on a second high density DNA array; and imaging said second high density DNA array using the detector, wherein the first sequencing reaction in (a) is initiated before the first sequencing reaction in (b) is initiated such that the sequencing reactions in (a) and (b) are staggered. By using asynchronous sequencing reactions and imaging two separate arrays using one detector, imaging can be carried out on one array while sequencing reactions are carried out on one the other, substrate, the other substrate is imaged, reducing the idle time of the imaging system.

DNA sequencing from high density DNA arrays using asynchronous reactions

Novel fluorescent nucleotide analogs are provided herein. Also provided herein are methods of using the nucleotide analogs in sequencing-by-synthesis and signal confinement methods.

Signal confinement sequencing (SCS) and nucleotide analogues for signal confinement sequencing

A high density DNA array comprising a patterned surface, said surface comprising a pattern of small DNA binding regions separated by a non-DNA binding surface, wherein the DNA binding regions comprise DNA capture chemistry and the non-DNA binding surface does not have the DNA capture chemistry wherein more than 50% of the DNA binding regions in the array have single informative DNA species.

High density DNA array

Growing community of inventors

Los Altos Hills, CA, United States of America

Snezana Drmanac