Danisco US Inc.

Dupont Nutrition Biosciences Aps

Nutrition & Biosciences USA 4, Inc.

E.i. Dupont De Nemours and Company

Rijksuniversiteit Groningen

Dupont Industrial Biosciences Usa, LLC

Slavko Kralj

Richard Ray Bott

Bradley R Kelemen

Thijs Kaper

Igor Nikolaev

Sander Van Stigt Thans

Johannes Franciscus Thomas Van Lieshout

Ronaldus Wilhelmus Hommes

Zheyong Yu

Zhenghong Zhang

Mats Sandgren

Maria Foukaraki

Mark Scott Payne

Frits Goedegebuur

Susan Marie Hennessey

Suzanne E Lantz

Yougen Li

Gudrun Vogtentanz

Ellen D Semke

Laurie A Howe

Veli Alkan

Jared B Parker

Wilhelmus Van Der Kley

Robert DiCosimo

David Aaron Estell

Qiong Cheng

Benjamin S Bower

Wolfgang Aehle

Louise Wallace

Jonathan Caspi

Marc Kolkman

Natnael Behabtu

Chris Leeflang

Lubbert Dijkhuizen

Ronaldus W J Hommes

Daniel Esteban Torres-Pazmino

Ronaldus Wilhelmus Joannes Hommes

Jonathan K Lassila

Johannis Paulus Kamerling

Paulien Kruithof

Johannes G De Nobel

Reinder Johannes Leemhuis

Arjen Hoekstra

Johan Van Lieshout

Wilhelmus Antonious Hendricus Van Der Kley

Justyna Malgorzata Dobruchowska

Marc Jos Elise Cornelis Van Der Maarel

Johan Franciscus Thomas Van Lieshout

Nicholai Douglas

Robert Dicosimo

An isolated and/or purified α-glucanotransferase from, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the α-glucanotransferase and variants thereof, host cells comprising the synthetic nucleic acids, and compositions comprising the α-glucanotransferase are provided. Methods of using the compositions include the manufacture of oligosaccharides.

Compositions and methods comprising the use ofandα-glucanotransferase enzymes

strain WDG185 expresses an inulosucrase that efficiently synthesizes a broad range of IOS with a GF range of GF3-GF30. The isolated and/or purified inulosucrase, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the inulosucrase, its variants, or its active fragments, host cells comprising the synthetic nucleic acids, and compositions comprising the inulosucrase are provided. Methods of using the compositions include the manufacture of inulooligosaccharides.

Compositions and methods comprising the use of ainulosucrase (INUO)

Reactions are disclosed herein comprising water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and an alpha-1,4-glucan phosphorylase. Novel alpha-1,4-glucan phosphorylase enzymes are also disclosed. Additional disclosures herein regard sucrose phosphorylase enzymes and methods of use thereof to produce alpha-G1P.

Alpha-glucose-1-phosphate synthesis from sucrose and glucan synthesis using glucan phosphorylases

Reaction compositions are disclosed herein comprising at least water, beta-glucose -1-phosphate (beta-G1P), an acceptor molecule, and an alpha-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with alpha-1,3 glycosidic linkages. Further disclosed are alpha-1,3-glucan phosphorylase enzymes and methods of use thereof.

Synthesis of glucan comprising alpha-1,3 glycosidic linkages with phosphorylase enzymes

Reaction compositions are disclosed herein comprising at least water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and a beta-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with beta-1,3 glycosidic linkages. Further disclosed are methods of isolating beta-1,3-glucan.

Synthesis of glucan comprising beta-1,3 glycosidic linkages with beta-1,3-glucan phosphorylase enzymes

An isolated and/or purified &#x3b1;-glucanotransferase from, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the &#x3b1;-glucanotransferase and variants thereof, host cells comprising the synthetic nucleic acids, and compositions comprising the &#x3b1;-glucanotransferase are provided. Methods of using the compositions include the manufacture of oligosaccharides.

Compositions and methods comprising the use of exiguobacterium acetylicum and bacillus coagluans alpha-glucanotransferase enzymes

Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and/or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.

Engineered glucosyltransferases

Reaction compositions are disclosed herein comprising at least water, beta-glucose-1-phosphate (beta-G1P), an acceptor molecule, and an alpha-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with alpha-1,3 glycosidic linkages. Further disclosed are alpha-1,3-glucan phosphorylase enzymes and methods of use thereof.

Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes synthesize alpha-glucan products having increased molecular weight. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.

Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described.

Variant enzymes

Variant Enzymes

Disclosed are a number of homologs and variants ofCe17A (formerlycellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.

Variants of cellobiohydrolases

The invention relates to the field of poly-and oligosaccharides and their nutritional effects. In particular, it relates to the application of &#x3b1;-glucanotransferases in methods for preparing dietary fibers, including prebiotic oligosaccharides, and to novel oligosaccharides obtainable thereby. Provided is a method for producing a mixture of gluco-oligosaccharides having one or more consecutive (&#x3b1;1&#x2192;6) glucosidic linkages and one or more consecutive (&#x3b1;1&#x2192;4) glucosidic linkages, comprising contacting a poly- and/or oligosaccharide substrate comprising at least two (&#x3b1;1&#x2192;4) linked D-glucose units with an &#x3b1;-glucanotransferase capable of cleaving (&#x3b1;1&#x2192;4) glucosidic linkages and making new (&#x3b1;1&#x2192;4) and (&#x3b1;1&#x2192;6) glucosidic linkages. Also provided are (isolated) gluco-oligosaccharides obtainable thereby, and their application in nutritional and cosmetic compositions.

Glucooligosaccharides comprising (alpha 1-&#x3e;4) and (alpha 1-&#x3e;6) glycosidic bonds, use thereof, and methods for providing them

Growing community of inventors

Oegstgeest, Netherlands

Slavko Kralj