Northeastern University

Novobiotic Pharmaceuticals, LLC

Slava Epstein

Kim Lewis

Dallas E Hughes

Aaron J Peoples

Losee Lucy Ling

Anthony Nitti

William Millett

Amy Spoering

Linos Lazarides

Victoria Alexandra Steadman

Michael Kenyon Jones

Karine Gaelle Poullennec

Jean-Yves Christophe Chiva

Kelly Demeo

Catherine Achorn

Alysha Desrosiers

Ashley Zullo

Edgar Daniel Goluch

Yoshiteru Aoi

Ekaterina Gavrish

Tammi Kaeberlein

Dallas Hughes

High capacity, low cost devices for use in growing monocultures of novel, previously unknown microbial species contain adhesive layers with wells covered by nanoporous membranes. The devices are placed in natural environments for cultivation of unknown microbial species.

Device for high throughput isolation and cultivation of microbial species

The present invention relates generally to novel depsipeptides, to methods for the preparation of the novel depsipeptides, to pharmaceutical compositions comprising the novel depsipeptides; and to methods of using the novel depsipeptides to treat or inhibit various disorders.

Depsipeptides and uses thereof

The present invention relates generally to novel depsipeptides, to methods for the preparation of these novel depsipeptides, to pharmaceutical compositions comprising the novel depsipeptides; and to methods of using the novel depsipeptides to treat or inhibit various disorders.

Depsipeptide and uses thereof

The invention provides devices and methods for automatically preparing pure cultures of microbial cells under native environmental conditions. The devices and methods permit the discovery and investigation of novel microorganisms having unknown culture requirements from natural environments and microbiomes. Cultures obtained using the invention lead to the identification and isolation of novel biologically active compounds. The devices utilize a single entry pore in a thin membrane to select a single cell for clonal expansion.

Method and device for cultivation and analysis of novel microbial species with unknown growth requirements

Nanofluidic devices and methods of the invention are capable of autonomously isolating individual microbial cells using constrictive channels and growing monocultures of the cells for automated characterization of their biochemical properties and interactions with mammalian cells. Single microbial cells, such as bacterial cells, are isolated directly from environmental sources and cultured using chemical factors from their native environment.

Nanofluidic device for isolating, growing, and characterizing microbial cells

Methods for producing a depsipeptide

Disclosed are methods of identifying compounds that suppress the growth of target cells. In certain embodiments, the methods comprise providing a panel of target cells and the panel of target cells comprises a plurality of populations of target cells. Each population comprises cells that are resistant to one or more compounds known to suppress the growth of cells sensitive to the one or more compounds. Each population of target cells grows in the presence of a candidate compound and a determination of whether the candidate compound suppresses the growth of one or more populations of target cells.

Methods of natural product drug discovery

Devices and methods for isolating and/or culturing microorganisms are provided. The devices comprise one or more semi-permeable membranes and may additionally include a growth medium for the microorganism. The devices and methods described herein can be used to isolate and culture both known and novel microorganisms from any environment.

Devices and methods for the selective isolation of microorganisms

The method of the invention is directed to the novel use of a diffusion chamber within which previously 'uncultivatible' microorganisms can be isolated. Rather than attempting to replicate the natural environment of an unknown microorganism, the method of the invention provides for exposing dividing microorganisms to all the components of the original environment while simultaneously containing the resulting colonies so that they can be isolated. The method of the invention can take advantage of the recognition that the preponderance of difficult-to-grow microorganisms do not form colonies visible to the naked eye. Therefore, these organisms must be isolated under a compound microscope as 'microcolonies.' In addition, methods according to the invention permit the isolation of novel microorganisms capable of growing in artificial media only in co-culture in the presence of a companion microorganism.

Isolation and cultivation of microorganisms from natural environments and drug discovery based thereon

Growing community of inventors

Dedham, MA, United States of America

Slava Epstein