Thermo Fisher Scientific Baltics Uab

Skaiste Valinskyte

Rasa Sukackaite

Remigijus Skirgaila

Renata Rimseliene

Mindaugas Ukanis

Martyna Simutyte

Karolis Matjosaitis

This disclosure relates to thermophilic family B DNA polymerases comprising a neutral amino acid residue at a certain position near the C-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type Pfu polymerase. The thermophilic family B DNA polymerases provided herein also comprise an N-terminal domain comprising a uracil-binding pocket that has been modified to reduce template uracil binding. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3'-5&#x2032; exonuclease domain and/or a sequence non-specific dsDNA binding domain.

Thermophilic DNA polymerase mutants

This disclosure relates to thermophilic family B DNA polymerases comprising a neutral amino acid residue at a certain position near the C-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type Pfu polymerase. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3'-5&#x2032; exonuclease domain and/or a sequence non-specific dsDNA binding domain.

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Vilnius, Lithuania

Skaiste Valinskyte