10X Genomics, Inc.

Dryco, Inc.

Siyuan Xing

Rajiv Bharadwaj

Augusto Manuel Tentori

Denis Pristinski

Hanyoup Kim

Felice Alessio Bava

Bill Kengli Lin

Cedric Uytingco

Preyas Shah

Rachel Foote

Nabil Mikhaiel

Pratomo Alimsijah

David Michael Patterson

Dieter Wilk

Erica Graziosa

Steven William Short

Provided herein is a fluid delivery method for permeabilizing a biological sample. The method includes delivering the fluid to a first substrate and/or a second substrate. At least one of the first substrate and the second substrate includes a spacer. The method further includes assembling, subsequent to the delivering, a chamber comprising the first substrate, the second substrate, the biological sample, and the spacer. The spacer may be disposed between the first substrate and second substrate. The spacer may be configured to maintain the fluid within the chamber and maintain a separation distance between the first substrate and the second substrate. The spacer may be positioned to at least partially surround an area on the first substrate on which the biological sample is disposed and/or at least partially surround the array disposed on the second substrate.

Fluid delivery methods

A method for aligning a sample to an array is provided. An image of sample image of a sample can be received by a data processor. The sample image having a first resolution. An array image including an overlay of an array with the sample and an array fiducial can be received by the data processor. The array image having a second resolution lower than the first resolution of the sample image. The sample image can be registered to the array image by aligning the sample image and the array image. An aligned image can be generated based on the registering. The aligned image can include an overlay of the sample image with the array. The aligned image can be provided by the data processor. A method for detecting fiducials associated with an array is provided. Systems and non-transitory computer readable mediums performing the method are also provided.

Sample handling apparatus and image registration methods

Quantitative methods for optimizing the permeabilization of cellular tissues for spatial transcriptomics are provided. Also provided is an instrument for quantitatively optimizing the permeabilization of cellular tissues used for spatial transcriptomics.

Quantitative and automated permeabilization performance evaluation for spatial transcriptomics

The present disclosure provides articles of manufacture with improved moisture control as well as methods of making such articles. In some embodiments, provided herein the article has a fabric with an outer hydrophobic surface and an inner surface with hydrophobic and hydrophilic regions where the hydrophilic regions can form a pattern, allowing moisture to collect and move through the pattern, and the hydrophobic regions prevent the whole inner surface from becoming moist and the outer hydrophobic regions does not show moisture. The fabric may be used to make garments. The entire inner surface of the garment may be patterned with hydrophilic patterns for partial absorption, and the entire outer surface of the garment is hydrophobic, thereby preventing perspiration from being seen from outside of the garment. Further provided herein are methods of making fabrics with improved moisture control including by printing or knitting.

Moisture control fabrics

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Newark, CA, United States of America

Siyuan Xing