Wisconsin Alumni Research Foundation

Siyoung Choi

William Leo Murphy

Xiaohua Yu

Disclosed are methods for cell transfection and regulating cellular behavior. More particularly, the present disclosure relates to methods of non-viral cell transfection and regulating cellular behavior using mineral coatings that allow for the enhanced transfection of cells. The mineral coatings bind polynucleotides and provide a source of calcium and phosphate ions to enhance transfection. The present disclosure also provides a high throughput platform for screening non-viral transfection of cells. The methods of the present disclosure also provide an advantageous polynucleotide delivery platform because the mineral coatings may be deposited on various medical device materials after being specifically developed using the high throughput screening platform.

Enhanced throughput mineral coatings for optimization of stem cell behavior

Controlled delivery of nucleic acid into target cells is achieved by immobilizing the nucleic acid and the cells to a substrate. Improved control over delivery is achieved by immobilizing the nucleic acid to the substrate via complementary DNA binding interactions with an oligonucleotide linker.

Growing community of inventors

Ithaca, NY, United States of America

Siyoung Choi