Dow Agrosciences LLC

Sivarama Reddy Chennareddy

Toby Cicak

David T Gillespie

Donald L McCarty, Ii

Rodrigo Sarria

Manju Gupta

Jyoti R Rout

Stephen N Novak

Dayakar Reddy Pareddy

Jayakumar Pon Samuel

Sean Michael Russell

Thomas J Parsons

Scott R Kaleyta

Liu Shen

Rodrigo Sarria-Milan

Pon Samuel Jayakumar

Tatyana Minnicks

Olga Karpova

David Griffin

Kelly Smith

Liu Yin Shen

The present disclosure relates in part to a method for identifying a soybean germline transformant from a population of soybean transformants by incorporating a selection agent within rooting medium used in tissue culture during the soybean transformation process. The soybean germline transformants are selected from a population of soybean transformants which are comprised of a combination of non-germline and germline soybean transformants. The soybean non-germline transformants are identified and eliminated early in the transformation process. The soybean germline transformants are identified and selected for culturing into mature soybean plants. The method is readily applicable for screening and obtaining a soybean germline transformant at an early stage in the soybean transformation process.

Soybean transformation method

A system and method for the automated or semi-automated preparation of seeds for transformation and transgenic engineering.

System for seed preparation and method of use

A system and method for the automated or semi-automated imagining and orienting of seeds to prepare the seeds for transformation and transgenic engineering.

System for imaging and orienting seeds and method of use

Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues using a disclosed bidirectional promoter fromorbidirectional constitutive promoter (BBCP). The constructs provided comprise at least one such bi-directional promoter linked to multiple gene expression cassettes, wherein each of the gene expression cassettes comprises at least one transgene. In some embodiments, the constructs and methods provided allow expression of genes between two and twenty.

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West Lafayette, IN, United States of America

Sivarama Reddy Chennareddy