Dsm IP Assets B.v.

Technische Universiteit Delft

Sipko Maarten Kuyper

Jacobus Thomas Pronk

Johannes Pieter Van Dijken

Wilhelmus Theodorus Antonius Maria De Laat

Aaron Adriaan Winkler

Wilhelmus Theordorus Antonius Maria De Laat

Adriaan Winkler Aaron

The present invention relates to genetic modifications in eukaryotic host cells that have been transformed to express a xylose isomerase that confers on the host cell the ability to isomerize xylose to xylulose. These genetic modifications are aimed at improving the efficiency of xylose metabolism and include. e.g., reduction of nonspecific aldose reductase activity, increased xylulose kinase activity and increased flux of the pentose phosphate pathway. The modified host cells of the invention are suitable for the production of a wide variety of fermentation products, including ethanol, in fermentation processes in which a source of xylose or a source of xylose and glucose are used as carbon source.

Metabolic engineering of xylose-fermenting eukaryotic cells

The present invention relates to further genetic modifications in eukaryotic host cells that have been transformed to express a xylose isomerase that confers the host cell the ability of isomerizing xylose to xylulose. The further genetic modifications are aimed at improving the efficiency of xylose metabolism and include e.g. reduction of unspecific aldose reductase activity, increased xylulose kinase activity and increased flux of the pentose phosphate pathway. The modified host cells of the invention are suitable for the production of a wide variety of fermentation products, including ethanol, in fermentation processes in which a source of xylose or a source of xylose and glucose are used as carbon source.

Xylose-fermenting yeast engineered to increase flux of the pentose phosphate pathway

Growing community of inventors

Delft, Netherlands

Sipko Maarten Kuyper