Hoffmann-la Roche Inc.

Simone Dethloff

Erhard Kopetzki

Stefan Seeber

Georg Tiefenthaler

Dominique Ostler

In the method as reported herein the isolation of nucleic acid segments encoding antibody variable domains and the insertion of the isolated nucleic acid segments in eukaryotic expression plasmids is performed without the intermediate isolation and analysis of clonal intermediate plasmids. Thus, in the method as reported herein the intermediate cloning, isolation and analysis of intermediate plasmids is not required, e.g. by analysis of isolated transformedcells.

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Bichl, Germany

Simone Dethloff