The University of Texas System

Simon Olivares

Laurence J N Cooper

Hiroki Torikai

Laurence Jn Cooper

Feng Wang-Johanning

Helen Huls

Ana Beatriz Korngold

Lenka Hurton

Brian A Rabinovich

Janani Krishnamurthy

Ling Zhang

Harjeet Singh

Radhika Thokala

Horoki Torikai

J N Laurence

Provided are methods of generating chimeric antigen receptors (CAR). In some embodiments, library screening of CAR is performed by generating a vector encoding the CAR from random attachment of vectors from libraries of vectors encoding antigen-binding domains (e.g., scFv regions), hinge regions, and endodomains. In some embodiments, the vectors contain a transposon.

Chimeric antigen receptors and methods of making

The present invention concerns methods and compositions for immunotherapy employing a modified T cell comprising a chimeric antigen receptor (CAR). In particular aspects, CAR-expressing T-cells are producing using electroporation in conjunction with a transposon-based integration system to produce a population of CAR-expressing cells that require minimal ex vivo expansion or that can be directly administered to patients for disease (e.g., cancer) treatment.

Human application of engineered chimeric antigen receptor (CAR) T-cells

Chimeric antigen receptor (CAR) polypeptides are provided comprising an antigen binding domain; a hinge domain; a transmembrane domain and an intracellular signaling domain, wherein the CAR polypeptide binds to a target antigen and wherein the antigen binding domain comprises HCDR sequences from a first antibody that binds to the target antigen and LCDR sequences from a second antibody that binds to the target antigen. In certain aspects, the target antigen is CD123.

Chimeric antigen receptor molecules and uses thereof

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Houston, TX, United States of America

Simon Olivares