Pixelgen Technologies Ab

Olink Proteomics Ab

Blueair Ab

Leland Stanford Junior University

Olink Ab

Agilent Technologies, Inc.

Simon Fredriksson

Ronald W Davis

Ulf Landegren

Olof John Ericsson

Mats Gullberg

Filip Karlsson

Henrik Johansson

Emma Rennel-Dickens

Martin Lundberg

Jakob Karl Mingettig

Toni Luukkonen Fredäng

Ryan Stephen Helps

Andrea Mambrini

Anna Eriksson

Christian Engene

Marie Charlotte Ideström

Anna Larsson

Carl Fredrik Victorin

Bonnie Tran

Carl Johan Anton Axelson

Carl Fredrik Nilsson

Mats Nilsson

Magnus Isaksson

Fredrik Roos

Air purifier

Provided herein is probe system comprising: a population of nucleic acid molecules that have an extendible end, a first set of barcoded particles that each have a nucleotide sequence comprising: (i) a binding sequence that is complementary to the extendible end of the nucleic acid molecules, (ii) a unique particle identifier sequence, and (iii) a first template sequence, and a second set of barcoded particles that each have a nucleotide sequence comprising: (i) the first template sequence and (ii) a unique particle identifier sequence. In use, extension of the nucleic acid molecules using the first set of barcoded particles of as a template produces extensions products that contain the complement of a unique particle identifier sequence of a particle and the complement of the first template sequence. Methods of using the probe system to map binding events in or on a cellular sample are also provided.

Spatial mapping by serial primer extension

Provided herein is a method comprising producing rolling circle amplification (RCA) products that are immobilized in or on cells, wherein each RCA product has an RCA product identifier sequence that distinguishes the RCA product from other RCA products that are immobilized in or on the cells; while the RCA products are immobilized in or on cells, producing nucleic acid products that contain RCA product identifier sequences or complements thereof from adjacent RCA products; for each of a plurality of the nucleic acid products, identifying which RCA product identifier sequences or complements thereof are in the nucleic acid product; and making a physical map of at least some of the RCA products using the identified RCA product identifier sequences or complements thereof.

Method for making a physical map of binding events that are on or on cells

Provided herein is a method for mapping rolling circle amplification (RCA) products that contain unique identifier sequences. The method generally involves (a) producing a complex comprising population of grid oligonucleotide molecules and a population of RCA products that each have a unique RCA product identifier sequence, wherein the grid oligonucleotides are hybridized directly or indirectly via a splint to complementary sites in the RCA products; (b) extending the grid oligonucleotide molecules that are hybridized to two RCA products to add the complements of the unique RCA product identifier sequences from the two RCA products to the grid oligonucleotide molecules; (c) sequencing the extended grid oligonucleotides; (d) analyzing the sequences to identify which pairs of unique RCA product identifier sequence complements have been added onto the grid oligonucleotides; and (e) making one or more physical maps of the immobilized RCA products using the pairs of sequences identified in (d).

Method for mapping rolling circle amplification products

Described herein is a complex comprising multiple nucleic acid molecules that are hybridized together, each comprising, from 5' to 3′, a first complementary sequence, a spacer sequence and a second complementary sequence, and either/or a 5′ end sequence that is 5′ of the first complementary sequence and terminates in a 5′ phosphate and a 3′ end sequence that is 3′ of the second complementary sequence and terminates in a 3′ hydroxyl. In this complex: the first complementary sequence of one molecule is directly or indirectly hybridized with the second complementary sequence of another molecule in the complex and the spacer sequence and the 3′ and/or 5′ end sequences are single-stranded. Methods of making and using the complex are also provided.

Rolling circle amplification product having multiple free ends

The present inventive concept relates to an air treatment device adapted to treat air in a confined space. The air treatment device comprises a device body having a central axis, the device body comprising a supporting structure being substantially transverse to the central axis and a side section being substantially parallel to the central axis. The air treatment device further comprises an air treatment section arranged in the device, an ionizing arrangement, a fan arrangement, and a filter extending in a first direction being parallel to the central axis, wherein the filter comprises an upper end and a lower end in the first direction, wherein the ionizing arrangement and the fan arrangement are arranged between the upper and lower end.

Air treatment device adapted to treat air in a confined space

The present invention relates to a proximity probe based detection assay ('proximity assay') for an analyte in a sample, specifically a proximity probe extension assay (PEA), an in particular to an improvement in the method to reduce non-specific 'background' signals, wherein the improvement comprises the use in such assays of a component comprising 3' exonuclease activity, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte; (b) allowing the nucleic acid domains of the proximity probes to interact with each other upon binding of said proximity probes to said analyte, wherein said interaction comprises the formation of a duplex; (c) contacting said sample with a component comprising 3&#x2032; exonuclease activity; (d) extending the 3&#x2032; end of at least one nucleic acid domain of said duplex to generate an extension product, wherein the step may occur contemporaneously with or after step (c); and (e) amplifying and detecting the extension product.

Exonuclease enabled proximity extension assays

The present invention relates to a proximity probe based detection assay ('proximity assay') for an analyte in a sample, specifically a proximity probe extension assay (PEA), an in particular to an improvement in the method to reduce non-specific 'background' signals, wherein the improvement comprises the use in such assays of a hyperthermophilic polymerase, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte; (b) allowing the nucleic acid domains of the proximity probes to interact with each other upon binding of said proximity probes to said analyte, wherein said interaction comprises the formation of a duplex; (c) extending the 3' end of at least one nucleic acid domain of said duplex to generate an extension product, wherein the extension reaction comprises increasing the temperature of assay above room temperature and uses a polymerase enzyme which is characterised as having less than 20% of its maximal enzyme activity at 40&#xb0; C. and having less than 10% of its maximal enzyme activity at 25&#xb0; C., wherein the optimum temperature for maximal activity of the polymerase is more than 40&#xb0; C. and wherein the polymerase is selected from(Pfu) DNA polymerase and(Pwo) DNA polymerase or a derivative or mutant thereof, preferably wherein said derivative is a sequence-modified derivative; and (d) amplifying and detecting the extension product.

Hyperthermophilic polymerase enabled proximity extension assay

The present invention relates to the use of a conjugate of a non-analyte-specific binding protein coupled to a nucleic acid as a blocking reagent in a probe-based detection assay, which uses a probe comprising a proteinaceous analyte-binding partner coupled to a nucleic acid domain to detect an analyte in a sample.

Blocking reagent and methods for the use thereof

Methods and compositions for detecting an analyte in a sample are provided. In practicing the subject methods, a sample is combined with at least a pair of proximity probes that each include an analyte binding domain and a nucleic acid domain. The resultant mixture is then contacted with a pair of asymmetric nucleic acid connectors. Proximity dependent connector mediated interaction between the nucleic acid domains of the proximity probes is then detected to determine the presence of the analyte in the sample. Also provided are kits and systems for practicing the subject methods.

Methods and compositions for use in analyte detection using proximity probes

The present invention relates to sensitive, rapid and convenient assays for detection and or quantification of one or more analyte(s) in solution using multivalent proximity probes. The proximity probes each comprise several binding moieties, such as antibodies, and associated nucleic acid(s). When the binding moieties have bound to their analyte(s), the nucleic acids on opposite proximity probes interact with each other and a signal is generated based on this interaction. The multivalent proximity probes are especially valuable for highly sensitive and specific protein detection.

Kit for proximity probing with multivalent proximity probes

Growing community of inventors

Stockholm, Sweden

Simon Fredriksson