Neste Oyj

Neste Oil Oyj

Valio Ltd.

Aalto University Foundation

Oy Alko Ab

Fortum Oyj

Ravintoraisio Oy

Simo Laakso

Ossi A Pastinen

Perttu Koskinen

Heidi Vainio

Mika Sipponen

Miia Mujunen

Tarja Suomalainen

Ilkka Lehtomaki

Ilkka Lehtomäki

Heidi Kahelin

Ville Pihlajaniemi

Timo Korpela

Annika Malm

Olavi Myllymaki

Annika Mäyrä-Mäkinen

Olavi Myllymäki

Marjatta Vahvaselkä

Soile Tynkkynen

Sanna Hokkanen

Miia Laamanen

Auli Rainio

Mauri Makela

Pekka Lehtinen

Ilkka LEHTOMÄKI

Olavi MYLLYMÄKI

The present disclosure relates to a method for obtaining sugar and lignin fractions from lignocellulosic materials and to a method for producing fermentation products using the sugars obtained from the lignocellulose. The present disclosure also relates to a method for improving the sugar yield in the enzymatic hydrolysate by introducing a pre-treatment step of hydrothermal hydrolysis with a delignification step using a cationic compound.

Method of processing lignocellulosic material using a cationic compound

The present disclosure relates to a method for obtaining sugar and lignin fractions from lignocellulosic materials and to a method for producing fermentation products using the sugars obtained from the lignocellulose. The present disclosure relates to a method for improving the sugar yield in the enzymatic hydrolysate by introducing a pre-treatment step of hydrothermal hydrolysis and an alkaline delignification step.

Method of processing lignocellulosic material using an alkaline delignification agent

The present disclosure relates to methods for producing microbial lipids. The present disclosure also relates to methods for producing microbial lipids using inhibitors obtainable from lignocellulosic materials to suppress the proliferation of unwanted microorganisms in the fermentation broth. The method can therefore reduce the risk of having contaminating microbes establish in the system and the cultivation and thus higher yields of microbial lipids may be obtained.

Method for producing single cell oil from lignocellulosic materials

The invention relates to a method for the recovery of oil from an aqueous solution containing oil and solids, as well as to the use of products obtained by this method. The method of the invention comprises at least the following steps of: Adjusting the oil- and solids-containing aqueous solution to a pH which is acidic, separating an oil- and solids-containing fraction from the acidic aqueous solution with an separation agent which is hydrophilic or assumes a hydrophilic character in response to water, recovering the oil- and solids-containing fraction separated by means of said separation agent, extracting oil away from the solids with a water-insoluble extraction agent, and recovering the oil-containing water-insoluble extraction agent.

Oil recovery method

The present invention relates to a method for the production of fat with a principal application as transportation biofuel or a component or raw material therefor. According to the method, cell masses, cell suspensions and/or liquid phases formed in the production of single cell oil, and/or biomass-containing side streams or microorganism cell masses for another purpose and/or originating from other sources, are contacted with a fat-production capable microorganism and the organism is allowed to produce fat. The resulting fat is recovered or the microorganism mass is passed to a single-cell oil production process. By means of the invention, the organic matter present in the cell mass and side streams of single-cell oil can be re-utilized for the production of the single-cell oil, thereby improving a total fat yield, as well as reducing an organic load of the side streams.

Method for the production of fat

The present invention relates to a process for producing enzymes and single cell oil. The process comprises that microorganisms capable of producing both single cell oil and enzymes are cultivated under conditions suitable for single cell oil production and enzyme production in a single cell oil production process. A microorganism culture comprising single cell oil and enzymes is obtained and at least part of the microorganism culture, of the supernatant and/or microorganism cells separated from the microorganism culture, of protein fraction enriched from the supernatant, and/or of protein fraction obtained from the cells is used as an enzyme preparation or as a source of enzymes. Single cell oil is recovered from the microorganism cells and used as biofuel, component of biofuel or as a starting material for biofuel production. Enzymes produced according to the process are used in the same or in another industrial process.

Process for producing enzymes

The present invention concerns a process for forming a fatty acid, a fatty acid ester or a mixture thereof from a soap-comprising starting material, in which process a metal-ion forming agent is added to the starting material, whereby a mixture is formed, which contains an insoluble phase and a liquid phase, the insoluble phase is separated from the liquid phase, and an acid is added into the insoluble phase to form a fatty acid, or a monohydric alcohol and an acid catalyst are added to form a fatty acid ester, whereby two phases are formed, an aqueous phase and an organic phase, or, first, an acid is added and then a monohydric alcohol and an acid catalyst are added into at least a portion of the formed fatty acid to esterify the fatty acid.

Production of fatty acid and fatty acid ester

The invention relates to a method for preparing conjugated linoleic acid and particularly its cis-9,trans-11 isomer from linoleic acid by fermentation. The invention also relates to products prepared by the method.

Method for preparing conjugated linoleic acid

This invention provides a simple and efficient technique for isolating and purifying alpha-, beta- and especially gamma-cyclodextrins, and also their chemical derivatives. The isolation of different cyclodextrin subforms is based on the use of specially designed macromolecular adsorbents. These adsorbents contain covalently bound ligands which are of suitable size to penetrate the internal cavity of the cyclodextrin molecules and form specific inclusion complexes with cyclodextrins. By means of this invention it is also possible to increase the proportion of any of the cyclodextrin subforms or its chemical derivative by removing the corresponding cyclodextrin subform with a specific sorbent.

Process for isolation and purification of cyclodextrins

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Turku, Finland

Simo Laakso