Universidad De Los Andes

Corporacion Corpogen

Silvia Restrepo Restrepo

Alvaro Mongui

Patricia Del Portillo Obando

Armando Junca Howard

A transposon (TnC_T7) was developed to partially supply the transcriptional machinery during functional analysis of genomic/metagenomic libraries. This transposon was conceived and constructed to have the ability to integrate randomly into any episomal DNA, allowing the inducible expression of the adjacent DNA regions in both directions. In general, this genetic tool included a kanamycin resistance gene, two bidirectional T7 promoters and the T7RNA polymerase-coding gene, the latter under the regulation of the inducible arabinose promoter (P). The experimental validation confirmed the TnC_T7 potential to be used in functional genomic/metagenomic studies, in order to partially overcome the limitations of the bacterial hosts, which prevent them to recognize most of foreign genes from DNA libraries.

Transposon that promotes functional DNA expression in episomal DNAs and method to enhance DNA transcription during functional analysis of metagenomic libraries

Growing community of inventors

Bogotá, Colombia

Silvia Restrepo Restrepo