Pharmacia & Upjohn S.p.a.

Pharmacia S.p.a.

Silvia Filippini

Anna Luisa Colombo

Leonid Fonstein

Umberto Breme

Sharee L Otten

Charles Richard Hutchinson

C Richard Hutchinson

Natalia Lomovskaya

Claudio Scotti

Natasha Lomovskaya

The present invention is directed to a process for improving daunorubicin and doxorubicin production by means of a recombinant microorganism in which a gene of daunorubicin metabolism is mutated. The mutated gene is preferably dnrU and/or dnrX.

Process for preparing doxorubicin

The present invention is directed to a process for improving daunorubicin and doxorubicin production by means of a recombinant microorganism in which a gene of daunorubicin metabolism involved in the glycosylation of daunorubicin to acid-sensitive, baumycin-like compounds is inactivated.

Process for preparing daunorubicin and doxorubicin

The present invention concerns a process for improving doxorubicin production by means of a recombinant Streptomyces peucetius strain bearing a mutation in the gene dnrU coding for a protein involved in the metabolism of daunorubicin.

The ability to improve the conversion of .epsilon.-rhodomycinone to daunorubicin can be conferred on a bacterial host transformed with a recombinant vector comprising a DNA coding for an appropriate protein useful for that conversion. Furthermore, the bacterial host may have a mutation that blocks the function of a gene of daunorubicin metabolism.

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Milan, Italy

Silvia Filippini