Kws Saat Se * Co. Kgaa

Silke Wieckhorst

Milena Ouzunova

Monika Kloiber-Maitz

Christof Bolduan

Britta Schulz

Markus Niessen

Frank Breuer

Nils Stein

Takayoshi Ishii

Andreas Houben

Jochen Kumlehm

Raheleh Karimi-Ashiyani

The present invention relates to non-transgenic and transgenic plants, preferably crop plants, having biological activity of a haploid inducer and comprising a polynucleotide which comprises a nucleotide sequence encoding a centromer histone H3 (CENH3) protein, wherein the polynucleotide comprises at least one mutation causing an alteration of the amino acid sequence of the CENH3 protein, and to a part of the part. Further, the invention provides methods of generating the inducer plants, methods of generating haploid and double haploid plants using the inducer plants as well as methods of facilitating cytoplasm exchange.

Generation of haploid plants

The present invention relates to non-transgenic and transgenic plants, preferably crop plants, comprising a mutation causing an alteration of the amino acid sequence in the CATD domain of the centromere histone H3 (CENH3), preferably within the loop1 or the α2-helix of the CATD domain, which have the biological activity of a haploid inducer. Further, the present invention provides methods of generating the plants of the present invention and haploid and double haploid plants obtainable by crossing the plants of the present invention with wildtype plants as well as methods of facilitating cytoplasm exchange.

Sorghum plants are provided which are capable of inducing haploidy by modifications in the genome related to a pollen-specific expressed patatin phospholipid producing haploid offspring and can be produced for hybrid breeding in short time by chromosome doubling inbred lines, that is, homozygous father and mother lines. In addition, methods are provided for producing transgenic and non-transgenic plant haploid inducers and improving the induction performance of plants.

Haploidization in sorghum

The present invention relates to non-tr&#xe4;nsgenic and transgenic plants, preferably crop plants, comprising a mutation causing an alteration of the amino acid sequence in the CATD domain of the centromere histone H3 (CENH3), preferably within the loop1 or the &#x3b1;2-helix of the CATD domain, which have the biological activity of a haploid inducer. Further, the present invention provides methods of generating the plants of the present invention and haploid and double haploid plants obtainable by crossing the plants of the present invention with wildtype plants as well as methods of facilitating cytoplasm exchange.

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Einbeck, Germany

Silke Wieckhorst