Helmholtz-Zentrum Fuer Infektionsforschung Gmbh

Universitaet Des Saarlandes

Sanofi

Universität Des Saarlandes

Helmholtz-Zentrum Fur Infektionsforschung Gmbh

Silke Wenzel

Joachim Wink

Rolf Wolfgang Hartmann

Rolf Mueller

Rolf Müller

Ronald O Garcia

Shwan Rachid

Katja Gemperlein

Luigi Toti

Mark Broenstrup

Wulf Dirk Leuschner

Johann Gassenhuber

Claudia Koenig

Tina Binz

Carsten Volz

Jan Henning Sahner

Hilda Sucipto

Matthias Groh

Caudia Koenig

Ronald Garcia

The invention relates to a process for producing one or more polyunsaturated fatty acids by means of heterologous gene expression comprising the steps of providing a production organism which comprises a heterologous gene cluster encoding a polyunsaturated fatty acid biosynthetic pathway encompassing a subsequence ER encoding an enoylreductase and a subsequence AT encoding an acyltransferase,

Production of fatty acids by heterologous expression of gene clusters from myxobacteria

The present invention refers to the gene cluster and genes comprised by the gene cluster which are involved in the biosynthesis of griselimycin and methylgriselimycin and to the use of the gene cluster, genes comprised thereby and proteins encoded thereby for the production of antibiotic agents.

Gene cluster for biosynthesis of griselimycin and methylgriselimycin

The invention provides the amino acid sequences comprised in or constituting the synthetic pathway enzymes participating in the production of Argyrins, as well as the nucleic acid sequences encoding the synthetic pathway enzymes participating in the production of Argyrins, as well as genetically manipulated microorganisms containing nucleic acid sequences encoding the synthetic pathway enzymes for the production of Argyrins, e.g. for inserting one or more of these coding sequences, mutating in a targeted manner one or more of these nucleic acid sequences, in a wild type producer micro-organism or in a heterologous micro-organism, for the production of Argyrins.

Synthetic pathway enzymes for the production of argyrins

The invention relates to a process for producing one or more polyunsaturated fatty acids by means of heterologous gene expression comprising the steps of providing a production organism which comprises a heterologous gene cluster encoding a polyunsaturated fatty acid biosynthetic pathway encompassing a subsequence ER encoding an enoylreductase and a subsequence AT encoding an acyltransferase, growing the production organism in the presence of a fermentable carbon source whereby one or more polyunsaturated fatty acids are produced, and optionally recovering the one or more polyunsaturated fatty acids.

The invention provides the amino acid sequences comprised in or constituting the synthetic pathway enzymes participating in the production of Argyrins, as well as the nucleic acid sequences encoding the synthetic pathway enzymes participating in the production of Argyrins, as well as genetically manipulated micro-organisms containing nucleic acid sequences encoding the synthetic pathway enzymes for the production of Argyrins, e.g. for inserting one or more of these coding sequences, mutating in a targeted manner one or more of these nucleic acid sequences, in a wild type producer micro-organism or in a heterologous micro-organism, for the production of Argyrins.

Growing community of inventors

Saarbruecken, Germany

Silke Wenzel