Hoffmann-la Roche Inc.

F. Hoffmann-la Roche Ag

Silke Hansen

Ralf Schumacher

Klaus-Peter Kuenkele

Dietmar Reusch

Erhard Kopetzki

Ulrich Goepfert

Hendrik Knoetgen

Oliver Ploettner

The invention provides an antibody comprising human IgG1 or IgG3 heavy chain constant domains that are glycosylated with a sugar chain at Asn297, said antibody being characterized in that the amount of fucose within said sugar chain is at least 99%, and in addition the amount of NGNA is 1% or less and/or the amount of N-terminal alpha 1,3 galactose is 1% or less, and uses thereof.

Glycosylated antibodies

The current invention comprises a nucleic acid encoding the amino acid sequence of the C-terminal part of the C3-domain of an immunoglobulin of the class IgA or IgG, or the C-terminal part of the C4-domain of an immunoglobulin of the class IgE or IgM, wherein the glycine-lysine-dipeptide comprised in the amino acid sequence of the C-terminal part of the C3- or C4-domain is encoded by the nucleic acid ggaaaa, or the nucleic acid ggcaaa, or the nucleic acid gggaaa, or the nucleic acid gggaag, or the nucleic acid ggcaag, or the nucleic acid ggaaag.

Heavy chain mutant leading to improved immunoglobulin production

Method for selecting CHO cell for production of glycosylated antibodies

Growing community of inventors

Iffeldorf, Germany

Silke Hansen