Boehringer Mannheim Gmbh

Siegfried Notzel

Manfred Baier

Dieter Mangold

Rolf Lerch

Gunter Pappert

Helmut Jering

Manfred Krause

Klaus P Kaspar

Rainer Schafer

Ellen Mossner

Peter Schafer

Ulrich Trager

Gerhard Schindler

Bernd Klein

Test carrier for the analysis of fluids with a frame (2) consisting of at least two parts surrounding a test field opening (6) and with a test field disposed in the test field opening (6). In order to provide such a test carrier with frame with which it is possible to assemble the test field out of several test layers manufactured separately from one another, it is proposed that the test field be designed as a test layer package (7) with test layers (10, 11, 12) resting loosely on one another, the frame (2) comprise two shaped pieces of plastics material, one of which serves as base part (4) and comprises a seat (16) for accommodating the test layers (10, 11, 12), while the other serves as lid part (3) and comprises a bearing surface (17) resting on the topmost layer (10) of the test layer package (7). The lid part (3) and the base part (4) are in addition designed with elastic properties so that the pressure with which the bearing surface (17) presses onto the test layer package (7) is substantially constant in the tolerance range of the thickness of the test field package (7).

Test carrier for analysis of fluids

A carrier fleece is prepared for use as reagent carrier from which reagents can be dissolved in as assay such as immunological analysis. The carrier fleece contains from 5 to 60% by weight of cellulose-containing fibers, from 40 to 95% by weight of polyester or polyamide polymer fibers or a combination thereof and from 5 to 30% by weight of the fibers of an organic binding agent which has a hydroxyl or an ester group or a combination thereof. In an immunological analysis, the carrier fleece is impregnated with an immunologically active agent such as a beta-galactosidase conjugate and then introduced into a solution of a sample containing an immunologically active substance to be analyzed. The immunologically active agent is eluted into the sample solution and the presence of the immunologically active substance in the sample is determined.

Carrier fleece for use as a reagent carrier from which reagents can be

The present invention provides a process for the preparation of a reagent paper for immunological analysis, wherein a fiber fleece of a cellulose/synthetic fiber mixture, in which the weight ratio of cellulose/synthetic fiber is 1 to 90/99 to 10, is activated by treatment with periodate, the so activated fiber fleece is loaded with an acid-treated protein and non-bound protein is removed. The present invention also provides a reagent paper for immunological analysis, wherein it comprises a fiber fleece of a cellulose/synthetic fiber mixture, in which the weight ratio of cellulose/synthetic fiber is from 1 to 90/99 to 10. Furthermore, the present invention is concerned with the use of a fiber fleece of a cellulose/synthetic fiber mixture, wherein the weight ratio of cellulose/synthetic fiber is 90 to 1/10 to 99, as reagent carrier for heterogeneous immunological analysis.

Reagent paper for immunologically analysis, a process for the

Growing community of inventors

Wilhelmsfeld, Germany

Siegfried Notzel