Corning Glass Works

Sidney Yaverbaum

Howard H Weetall

Ralph A Messing

Leroy S Hersh

William P Vann

Biologically active proteins such as enzymes and antibodies can be chemically bonded to a variety of high surface area, inorganic supports by reacting the support surface with polymeric isocyanates and then reacting the coated surface with a dispersion of the proteins.

Immobilization of proteins on inorganic support materials

Method of detecting and/or quantitating anti-virus antibodies comprising the steps of reacting serum containing an unknown amount of a given antibody, a known amount of the antibody (radioactively labelled), and a composite consisting of the antibodies coupled chemically through an intermediate silane coupling agent to a porous glass body, the composite having complexed thereto quantities of the virus; separating the composite from the reaction solution after a suitable reaction period; determining the radioactivity count of the separated composite or the remaining solution; and, relating the count to a standard curve.

Detection and quantitation of viral antibodies

Method of determining the concentration of a substance in a solution which comprises the steps of incubating the solution with a known amount of radioactively labelled substance and a suspendable composite consisting of anti-substance antibody coupled chemically through an intermediate silane coupling agent to an inorganic carrier, the incubation period being for a time sufficient to form immunochemical complexes, separating the complexes from the solution, determining the radioactivity of either the separated complexes or the remaining solution, and relating that determination to a standard curve. Specific assays for digoxin, insulin, and estriol are disclosed.

Solid phase radioimmunoassay

Clinically significant concentrations of digoxin can be determined by reacting an unknown amount of digoxin, a known amount of labelled digoxin, and a composite consisting of anti-digoxin antibodies coupled through an intermediate silane to magnetically responsive particles; magnetically separating the immunochemical complexes formed thereby, counting the radioactivity of either the separated products or remaining solution, and relating the count to a standard curve.

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Big Flats, NY, United States of America

Sidney Yaverbaum