Pfizer Corporation

Zoetis Services LLC

Zoetis LLC

Pharmacia & Upjohn Company

Pfizer Products Inc.

Zoetis P&u LLC

Zoetis P LLC

Siao-Kun Wan Welch

Jay Gregory Calvert

Michael G Sheppard

David Ewell Slade

Shelly Lynn Shields

Raymond R R Rowland

Dal-Young Kim

Robert G Ankenbauer

Xuemei Cao

Dongwan Yoo

Changhee Lee

Michael K O'Hara

Nancee Lois Oien

Yugang Luo

Ying Yuan

Michael G Shepard

Lynn D Nelson

Michael George Sheppard

The invention provides isolated polynucleotide molecules that comprise a DNA sequence encoding an infectious RNA sequence encoding a genetically-modified North American PRRS virus, methods to make it and related polypeptides, polynucleotides, and various components. Vaccines comprising the genetically modified virus and polynucleotides and a diagnostic kit to distinguish between naturally infected and vaccinated animals are also provided.

North American porcine reproductive and respiratory syndrome (PRRS) virus and uses thereof

The present invention relates to chimeric pestiviruses having utility as immunogenic compositions and vaccines. Also described herein are methods and kits for treating or preventing the spread of bovine viral diarrhea virus infection, as well as methods and kits for differentiating between vaccinated and wild-type infected animals.

Chimeric pestiviruses

The present invention relates to improved diagnostic methods and kits for differentiating between (a) animals administered a chimeric pestivirus, and (b) animals infected with a wild-type bovine viral diarrhea virus (BVDV) or immunized with a conventional BVDV vaccine.

Vaccine diagnostics

The present invention provides methods and compositions related to the generation of host cells permissive for virus growth, particularly Porcine Reproductive and Respiratory Syndrome (PRRS) virus.

Cellular permissivity factor for viruses and uses thereof

The invention provides isolated polynucleotide molecules that comprise a DNA sequence encoding an infectious RNA sequence encoding a genetically-modified North American PRRS virus, wherein the polynucleotide molecule lacks at least one detectable antigenic epitope of North American PRRS virus. The invention also provides vaccines comprising genetically modified North American PRRS virus, RNA molecules, plasmids and viral vectors comprising the isolated polynucleotide molecules. Also provided are isolated polynucleotide molecules further comprising at least one nucleotide sequence that encodes a detectable heterologous antigenic epitope, and vaccines comprising North American PRRS virus, RNA molecules, plasmids and viral vectors comprising such isolated polynucleotide molecules.

Infectious cDNA of north american porcine reproductive and respiratory syndrome (PRRS) virus and uses thereof

Infectious cDNA clone of North American porcine reproductive and respiratory syndrome (PRRS) virus and uses thereof

The invention provides isolated polynucleotide molecules, plasmids, viral vectors, and transfected host cells that comprise a DNA molecule encoding an infectious RNA molecule that encode a PRRS (Porcine Reproductive and Respiratory Syndrome) virus, and further provides the PRRS virus encoded thereby. The invention also provides isolated infectious RNA molecules encoding PRRS virus.

Infectious cDNA clone of north american porcine reproductive and respiratory syndrome (PPRS) virus and uses thereof

The invention provides isolated polynucleotide molecules, including plasmids; viral vectors; and transfected host cells that comprise a DNA sequence encoding an infectious RNA sequence encoding a North American PRRS virus; and also North American PRRS viruses encoded thereby. The invention further provides isolated infectious RNA molecules encoding a North American PRRS virus. The invention also provides isolated polynucleotide molecules, infectious RNA molecules, viral vectors, and transfected host cells encoding genetically-modified North American PRRS viruses; and genetically-modified North American PRRS viruses encoded thereby. The invention also provides vaccines comprising such plasmids, RNA molecules, viral vectors, and North American PRRS viruses, and methods of using these vaccines in swine and in other animals. Also provided are isolated polynucleotide molecules, viral vectors, and transfected host cells that comprise a nucleotide sequence encoding a peptide of a North American PRRS virus. These viral vectors and transfected host cell lines are useful in providing peptides to compensate for mutated peptide coding sequences of DNA sequences encoding genetically-modified North American PRRS viruses so that functional virions can be generated.

The present invention provides a genetically modified PRRS virus, methods to make it and related polypeptides, polynucleotides and various components. Vaccines comprising the genetically modified virus and polynucleotides are also provided.

N protein mutants of porcine reproductive and respiratory syndrome virus

The present invention provides safe vaccines and methods of preparing such vaccines. The vaccines of the present invention contain at least two live mutant viruses of the same family or nucleic acid molecules encoding such viruses, wherein each of the two viruses or the encoding nucleic acids contains a mutation that confers a desirable phenotype and the mutations in the viruses reside in the same genomic site such that the mutant viruses cannot recombine with each other to eliminate the mutations.

Growing community of inventors

Kalamazoo, MI, United States of America

Siao-Kun Wan Welch