Genotech Corp.

Intron Biotechnology, Inc.

Si Kyu Lim

Jae Jong Kim

Dong Hwan Kim

Yeo Joon Yoon

Sang Myoun Lim

Bo-mi Lee

Mi Ok Lee

Jeoung Hyun Ryu

Keum Soon Lee

Sun Ho Cha

Bo-Mi Lee

The present invention relates to a method for detecting single nucleotide polymorphism (SNP) using a feature that the 5'-flap endonuclease (FEN) activity of DNA polymerase is inhibited when a probe complementarily binds to the end of a polymerase chain reaction (PCR) product. More specifically, the present invention relates to a novel method wherein it was verified that, when a probe used for a real-time PCR complementarily binds to the end site of a PCR product, the 5&#x2032;-FEN activity of thermostable DNA polymerase to the probe is inhibited, and thus when such a feature is used to make a design such that an SNP site to be detected is located at the 5&#x2032;-end site of the probe, the 5&#x2032;-flap formation is induced according to the allele, thereby allowing effective SNP detection.

Method for testing a mutant gene through real time polymerase chain reaction using inhibition of 5'-flap endonuclease activity

The present invention relates to novel tacrolimus analogs, a composition for the prevention or treatment of neurological diseases or immune hypersensitivity disorders comprising the same, a method for preventing or treating neurological diseases or immune hypersensitivity disorders comprising administering the analogs to a subject, a method for preparing the analogs using an isolated modifiedsp. strain wherein the activity of one or more enzymes selected from the group consisting of TcsA, TcsB, TcsC and TcsD is reduced; and the isolated modifiedsp. strain for prepare the analogs.

Tacrolimus analogues, a neuroprotective composition comprising the same, an immunosuppressive composition comprising the same, a method for preparing the same, and a mutant for producing the same

The present invention relates to a method for separating and purifying a lactone compound with an unsaturated alkyl group from a lactone compound with a saturated alkyl group which is an analog thereof. More specifically, the present invention relates to a method for effectively separating a lactone compound with an unsaturated alkyl group such as FK506 at high purity by extraction with a silver ion (Ag) solution without use of column chromatography.

Purification method of lactone compounds containing unsaturated alkyl group by extraction with silver ion solution

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Daegu, South Korea

Si Kyu Lim