National Chung Hsing University

Other

Shyi-Dong Yeh

Yi-Jung Kung

Tsung-Chi Chen

Chung-Chen Fan

Shu Chen

Tien-Tsai Su

Huey-Jiunn Bau

Ying-Huey Cheng

Kuan-Chun Chen

Fang-Lin Liu

Ya-Ling Huang

Chao-Hsiu Hsuan Yuan

Ching-Fu Yang

Hao-Wen Cheng

Yan-Wen Kuo

Hui-Chin Wang

Shin-Lan Wang

Ching-Wen Huang

Hei-Tu Hsu

Provided is a strategy for generating transgenic plants with concurrent resistance to DNA and RNA viruses at one construction, so as to develop an RNA-directed DNA methylation (RdDM) transgenic system using a hairpin construct of Ageratum yellow vein virus (AYVV) promoter region residing in an intron to resist DNA virus infection by RdDM. Furthermore, the hairpin construct of the AYVV promoter region coupled with an untranslatable nucleocapsid protein (NP) fragment of Melon yellow sport virus (MYSV) is created to induce post-transcriptional gene silencing (PTGS) against MYSV. A method for providing transgenic plants conferring concurrent resistance to both AYVV and MYSV for control of DNA and RNA virus at the same time, and underlying RdDM and PTGS mechanisms, respectively, is also provided.

Recombinant construct, recombinant microorganism, recombinant plant cell and method of providing plant with resistance against DNA virus and RNA virus

Provided are a eukaryotic expression system and its applications. The eukaryotic expression system has a recombinant plant cell. The recombinant plant cell includes a first vector and a second vector. The first vector expresses a fusion protein containing an Asia tospoviral common epitope. The fusion protein containing Asia tospoviral common epitope consists of an amino acid sequence as set forth in SEQ ID NO. 1, and a predetermined protein fragment connecting to the Asia tospoviral common epitope. The above eukaryotic expression system is useful for monitoring the interaction between proteins via use of a specific peptide to tag the predetermined protein and demonstrates high sensitivity and stability.

Eukaryotic expression system and use thereof

Provided is an isolated nucleic acid molecule having a right border flanking region, a left border flanking region and a transgene sequence between the right border flanking region and the left border flanking region, wherein the right border flanking region having at least 90% homology with the sequence set forth in SEQ ID NO: 29; the left border flanking region having at least 90% homology with the sequence set forth in SEQ ID NO: 31; and the transgene sequence having a papaya ringspot virus coat protein gene and a promoter operably linked to the papaya ringspot virus coat protein gene. Primers, probes and kit derived from the isolated nucleic acid molecule are proved to be useful for identifying the transgenic papaya line 16-0-1 in a specific, reproducible, sensitive and reliable way.

Isolated nucleic acid molecules from transgenic papaya line 16-0-1 resistant to papaya ringspot virus and use thereof

Provided is a recombinant plasmid having a control sequence and a coding sequence fragment of Papaya ringspot virus (PRSV) helper-component protease gene (HC-Pro gene) operably linked to the control sequence. A recombinant microorganism derived therefrom is also provided. A method for providing plants with resistance against virus is also provided. Use of PRSV HC-Pro gene or fragment thereof in generating plants with resistance against virus is also provided. It is proven that the PRSV HC-Pro transgenic plants can solve the problem resulting from breakdown by gene silencing suppression and provide broad-spectrum resistance to various PRSV strains of different geographical origins.

Gene-transfer vector comprising helper-component protease gene of papaya ringspot virus for broad-spectrum virus resistance in crops and use thereof

Provided is an isolated nucleic acid molecule having a right border flanking region, a left border flanking region and a transgene sequence between the right border flanking region and the left border flanking region, wherein the right border flanking region having at least 90% homology with the sequence set forth in SEQ ID NO: 30; the left border flanking region having at least 90% homology with the sequence set forth in SEQ ID NO: 32; and the transgene sequence having aringspot virus coat protein gene and a promoter operably linked to theringspot virus coat protein gene. Primers, probes and kit derived from the isolated nucleic acid molecule are proved to be useful for identifying the transgenicline 18-2-4 in a specific, reproducible, sensitive and reliable way.

Isolated nucleic acid molecules from transgenic papaya line 18-2-4 resistant to papaya ringspot virus and use thereof

The invention is an assay for detection of Watermelon silver mottle virus (WSMoV)-serogroup tospoviruses using a monoclonal antibody and a method for preparing the monoclonal antibody. A hybridoma cell line that produces a monoclonal antibody against the NSs proteins of WSMoV-serogroup tospoviruses was produced. The hybridoma cell line produces a monoclonal antibody binding with peptide SEQ ID No. 19.

Monoclonal antibody to the common epitope of NSs protein of watermelon silver mottle virus and assay for tospovirus

A method of using a tospoviral nucleic acid molecule of the sequence of nt (nucleotide) 3975-4928 in accordance with GenBank Accession No. AF133128 or a full complement thereof comprising the steps of: (a) obtaining at least one fragment made from the tospoviral nucleic acid molecule; (b) obtaining a transgene from the at least one fragment; (c) introducing the transgene into a plant to generate a transgenic plant; (d) culturing the transgenic plant; (e) selecting a transgenic plant with broad-spectrum resistance; and (f) obtaining the transgenic plant with broad-spectrum resistance.

Use of a tospoviral nucleid acid molecule for broad-spectrum transgenic resistance against different tospoviruses

Growing community of inventors

Taichung, Taiwan

Shyi-Dong Yeh