Toyota Jidosha Kabushiki Kaisha

Kyoto University

Shusei Obata

Masayoshi Muramatsu

Tanetoshi Koyama

Tokuzo Nishino

Kyozo Ogura

Yoshihiro Sato

Ayumi Koike

Sakayu Shimizu

Chikara Ohto

Masakazu Ito

Katsunori Kohda

Hiromi Kambe

Takashi Shimamura

Seiichi Taguchi

Satoshi Yoneda

Jun Ogawa

Shigenobu Kishino

Tomohisa Kuzuyama

Shinichi Ohnuma

Kazuhiko Totsuka

Kenji Tajima

Yasuharu Satoh

Kiyohiko Nishi

Katsuhiro Ohno

Ken'ichiro Matsumoto

Masahiro Sugimura

Miwa Yamada

Ai Inoue

Ayumi Takeshita

Takeshi Nakazawa

The present disclosure provides a novel aldehyde synthase gene having activity of synthesizing a medium-chain aldehyde. Such gene encodes a protein comprising the amino acid sequence as shown in SEQ ID NO: 2.

Aldehyde synthase gene, recombinant microorganism comprising the same, and method for producing alkane using the same

The present disclosure is intended to identify a substitution mutation that improves enzyme activity of a decarbonylase. An amino acid corresponding to an amino acid 90, 107, 163, or 171 in SEQ ID NO: 2 is to be substituted with an amino acid exhibiting a higher degree of hydrophobicity.

Mutant decarbonylase gene, recombinant microorganism comprising the mutant decarbonylase gene, and method for producing alkane

This invention relates to a recombinant microorganism having improved butanol production capacity and butanol production with the use of such recombinant microorganism with good efficiency. In this invention, the acetoacetyl-CoA synthase gene encoding an enzyme capable of synthesizing acetoacetyl-CoA from malonyl-CoA and acetyl-CoA and a group of genes involved in butanol biosynthesis that enables synthesis of butanol from acetoacetyl-CoA are introduced into a host microorganism.

Recombinant microorganism having butanol production capacity and butanol production method

A method of producing a prenyl alcohol(s) by culturing a mutant cell into which a fusion gene of farnesyl diphosphate synthase gene and geranylgeranyl diphosphate synthase gene has been introduced and recovering the prenyl alcohol(s) from the resultant culture.

Process for producing prenyl alcohols

It is an object of the present invention to provide a method for efficiently producing a polylactate via microbial fermentation with the use of a sugar as a starting material. The method is a method for producing a polylactate, which comprises the steps of:

Method for production of polylactate using recombinant microorganism

The object of the present invention is to provide a method for efficiently producing a polyester copolymer consisting of 3-hydroxybutyrate and lactate via microbial fermentation with the use of a sugar as a starting material. The method may comprise culturing a recombinant microorganism expressing: a protein capable of catalyzing the transfer of CoA to propionic acid and/or lactate; a protein capable of catalyzing the formation of acetoacetyl-CoA from two acetyl-CoA molecules; a protein capable of catalyzing acetoacetyl-CoA reduction; and a protein capable of catalyzing polyhydroxyalkanoate synthesis. According to the production method, a polyester copolymer consisting of 3-hydroxybutyrate and lactate can be efficiently produced using an inexpensive carbon source as a starting material, and thus the production cost of a biodegradable plastic can be reduced.

Method for production of polyester copolymer using recombinant microorganism

This invention provides a recombinant yeast that can produce branched alcohol appropriate for automotive fuel and the like and a branched alcohol production method whereby branched alcohol can be produced at low cost with the use of the recombinant yeast. A recombinant yeast in which a hydroxymethyl glutaryl-CoA reductase gene has been expressed to a high degree and the ADP-ribose pyrophosphatase gene and/or the yhfR gene are introduced so as to be expressed therein is provided.

Recombinant yeast and branched alcohol production method using recombinant yeast

A method of producing a prenyl alcohol, comprising creating a recombinant by transferring into a host a recombinant DNA for expression or a DNA for genomic integration each comprising a prenyl diphosphate synthase gene or a mutant thereof, culturing the resultant recombinant, and recovering the prenyl alcohol from the resultant culture.

Methods of producing prenyl alcohols

The present invention provides a high production method of prenyl alcohol, which comprises culturing prenyl alcohol-producing cells in a medium with an increased sugar content in the presence of at least one member selected from the group consisting of a surfactant, a fat or oil, and a terpene to produce and accumulate prenyl alcohol in the cells; allowing the accumulated prenyl alcohol to be secreted from the cells; and then collecting prenyl alcohol. The present invention enables prenyl alcohol to be highly produced in and effectively secreted from prenyl alcohol-producing cells by culturing the cells in a medium with an increased sugar content in the presence of at least one member selected from the group consisting of a surfactant, a fat or oil, and a terpene.

High production method of prenyl alcohol using saccharomyces cells

The present invention provides a method for producing geranylgeraniol and analogous compounds thereof, which comprises culturing yeast cells (ascomycetes and deuteromycetes), bacterial cells, actinomycete cells or filamentous fungus cells, all of which are capable of producing geranylgeraniol and analogous compounds thereof, in a medium to produce and accumulate geranylgeraniol and analogous compounds thereof in the cells and/or in the extracellular environment; and then collecting these compounds. The present invention enables inexpensive mass production of geranylgeraniol and analogous compounds thereof by using microorganisms capable of producing geranylgeraniol, farnesol and/or nerolidol useful as biosynthetic intermediates of terpenes, carotenoids and/or steroids.

Method for production of geranylgeraniol and analogous compounds thereof by microorganisms

The present invention provides a prenyl diphosphate synthetase and a gene coding for the synthetase. The invention discloses a recombinant protein having the amino acid sequence shown in SEQ ID NO:2 or a recombinant protein which has the amino acid sequence shown in SEQ ID NO:2 having deletion, substitution or addition of at least one amino acid and which has decaprenyl diphosphate synthetase activity; a gene coding for the protein; a recombinant vector comprising the gene; a transformant transformed with the vector; a method for producing a decaprenyl diphosphate synthetase; and a method for producing ubiquinone-10.

Decaprenyl diphosphate synthetase gene

Heptaprenyl diphosphate (HDP)-synthetase derived from Bacillus stearothermophilus which enzymes have the amino acid sequences shown as SEQ ID NOs: 1 to 3; 1 and 2; 2 and 3; or 1 and 3, DNA encoding them, and a method of producing the enzymes. According to the invention it is possible to industrially produce HDP-synthesizing enzyme and HPD.

Heptaprenyl diphosphate-synthetase

A DNA sequence that encodes a stable farnesyl pyrophosphate synthetase and the use thereof are provided. DNA sequence that encodes a farnesyl pyrophosphate synthetase originating from Bacillus stearothermophilus is provided. By selecting Bacillus stearothermophilus as the gene origin of the synthetase, a production system for the synthetase, a production system for the synthetase particularly having thermal stability, can be constructed.

Growing community of inventors

Nagoya, Japan

Shusei Obata