Rohm Co., Ltd.

Shun Momose

Akinori Yokogawa

Toshihiro Mori

Hidetaka Okada

Hiroki Takeuchi

Yasuhisa Kageyama

Youichi Aoki

Yoko Hayashi

A microchip including a fluid circuit therein and a specimen inlet for introducing a specimen containing a first component and a second component different in specific gravity from each other into the fluid circuit is provided, in which the fluid circuit includes a specimen measurement unit connected to the specimen inlet and having a prescribed volume for measuring the specimen introduced through the specimen inlet and a separation unit which is a site connected to the specimen measurement unit and having a capacity capable of storing the total amount of the measured specimen, for storing the total amount of the measured specimen and separating the first component and the second component in the stored specimen from each other.

Microchip

A microchip including a fluid circuit composed of a space formed inside is provided. The space includes a first space, a second space, and a space connecting portion connecting the first space and the second space, and the space connecting portion has a structure portion restraining liquid moving between the first space and the second space from moving due to wettability with respect to the space connecting portion surface.

A microchip to be used for measuring a plurality of types of objects to be measured. The microchip includes at least a reagent retaining portion and a detecting portion. The test reagent retaining portion includes a plurality of types of test reagents corresponding respectively to the plurality of types of objects to be measured. A plurality of time courses for a change in detected value at the detecting portion caused by a reaction between the test reagents and the objects to be measured corresponding respectively thereto are all different from each other.

Microchip, measurement system and method using the same, and test reagent to be used for microchip

Provided is a liquid reagent containing microchip having a fluid circuit formed of a space inside thereof. Liquid present in the fluid circuit is transferred to a desired position in the fluid circuit by applying centrifugal force. The fluid circuit includes a reagent retaining portion for accommodating a liquid reagent. The microchip includes an air introduction path formed of a groove provided on an outer surface of the microchip and coupled to the reagent retaining portion for introducing air into the reagent retaining portion, and a sealing portion provided so as to be detachable from the microchip for sealing the air introduction path. A method of using the microchip and a packaged liquid reagent containing microchip using the microchip are also provided.

Liquid reagent containing microchip and method of using the same, and packaged liquid reagent containing microchip

A microchip which includes a fluid circuit defined by a space formed in the microchip and migrates a liquid present in the fluid circuit to a desired position in the fluid circuit by an applied centrifugal force, and a movement path control region (a surface region where an uneven pattern is formed on an inner surface of the fluid circuit) for controlling a movement path of the fluid.

Provided are a microchip including a fluid circuit consisting of a space formed therein, a light-absorbing first substrate and a light-transmitting second substrate bonded onto the first substrate, and a groove, having a V-shaped section, extending parallelly to the fluid circuit on a surface of the second substrate opposite to the first substrate on a position immediately above at least part of the fluid circuit and a method of manufacturing the same.

Microchip and method of manufacturing the same

There are provided a microchip provided with a liquid reagent retaining portion that retains the liquid reagent, and a usage thereof, wherein the liquid reagent is sealed into the liquid reagent retaining portion by a sealant inactive to the liquid reagent, exhibiting flowability at the time of using the microchip, and the microchip further has a separating portion to separate the liquid reagent and the sealant, connected to the liquid reagent retaining portion, and the separating portion is composed of a separation tank for separating the liquid reagent and the sealant, and an accommodation tank for accommodating a separated substance, connected to the separation tank by a first flow path.

Microchip and method of using microchip

A microchip having a fluid circuit therein for passing a liquid is provided, wherein the fluid circuit has a first reservoir and a second reservoir for storing at least a part of the liquid, a first path connecting the first reservoir and the second reservoir, and a second path connecting the first reservoir and the second reservoir at a position different from the first path, and the first reservoir, the second reservoir, the first path, and the second path constitute a circular path capable of circulating the liquid.

A fabrication method of a microfluidic circuit is provided, dispensable of mask registration, absent of deviation in mask positioning, and inexpensive in fabrication cost. The fabrication method of a microfluidic circuit has a light transmissive substrate stacked on a light absorptive substrate, and the light transmissive substrate welded with the light absorptive substrate for bonding by directing light through the light transmissive substrate. The microfluidic circuit includes a microchannel at the bottom face of the light transmissive substrate and/or at the top face of the light absorptive substrate. The method includes the step of forming, at the light transmissive substrate, a light attenuation region attenuating transmittance of light towards the microchannel when light is directed through the light transmissive substrate.

Fabrication method of microfluidic circuit, and microfluidic circuit fabricated by method thereof

There is provided a microchip having an introduction portion introducing a liquid, a valve, and an ejection portion ejecting the liquid. The valve connects the introduction portion and the ejection portion, and retains the liquid such that surface tension of the liquid prevents the liquid from being ejected to the ejection portion. The liquid can be ejected from the introduction portion to the ejection portion by applying to the liquid centrifugal force larger than the surface tension of the liquid. Such a microchip according to the present invention has a simple configuration and allows easy control of retaining and flowing of a liquid.

Microfluidic circuit

A microchip includes fluid circuits therein, formed by uniting together at least a first substrate that is a transparent substrate and a second substrate having grooves provided at the substrate surface and/or through holes penetrating in a thickness direction. The fluid circuits include a liquid reagent receptacle unit to store a liquid reagent, a quantification unit to quantify the liquid reagent or specimen, and an overflow liquid storage unit connected to the quantification unit to store the liquid reagent or specimen overflowing from the quantification unit during quantification. There is also provided a method of using the microchip.

Microchip and method of using the same

A microchip is provided, in which dead space provided only for holding excess fluid is made smaller. The microchip is formed by joining at least a first substrate with a trench formed on the substrate surface and a second substrate, and it has a fluid circuit formed by the trench and a surface of the second substrate facing the first substrate. The first substrate and/or the second substrate has a projection for moving fluid and/or air in a direction opposite to the direction of gravity during an operation of the microchip, and the projection is provided near an end portion of a through hole and/or an air vent.

A microchip formed by joining a first substrate having at least one recess on its surface and a second substrate, wherein small projections of 0.5 to 30 &#x3bc;m in height are formed on at least a part of the surface having the recess of the first substrate, and a coating formed of a surface processing agent is provided on at least a part of the surface having the small projections formed thereon, as well as a method of manufacturing the microchip, are provided. A microchip allowing easy inspection of the state of application or state of adhesion of liquid material such as a surface processing agent, and allowing accurate optical measurement without causing disturbance such as fluorescence, can be provided.

Microchip and method of manufacturing microchip

The present invention provides a microchip which is made by bonding a first substrate having a groove provided at the substrate surface and a second substrate together, and has a fluid circuit therein, the fluid circuit having a separation portion for separating a first component, and a groove which constitutes the separation portion including an approximately V-shaped region surrounded by prescribed flow channel walls. At a top of the separation portion, a flow rate limiting portion limiting a flow rate of a fluid is preferably provided.

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Kyoto, Japan

Shun Momose