Seikagaku Corporation

Seikagaku Kogyo Kabushiki Kaisha

Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation)

Shun-ichiro Kawabata

Toshio Oda

Hikaru Mizumura

Sadaaki Iwanaga

Tetsu Saito

Yuki Kobayashi

Provided is a technology related to a horseshoe crab factor B variant, and also provided is means for performing endotoxin measurement with high sensitivity. A polypeptide having an amino acid sequence in which the amino acid residue at the 193-position in an amino acid sequence of a polypeptide of horseshoe crab factor B is substituted with a cysteine (Cys) residue, is produced. Endotoxin measurement can be carried out with high sensitivity by configuring areagent by combining this polypeptide with horseshoe crab factor C.

Horseshoe crab factor B variant

A horseshoe crab Factor C protein having activity of Factor C, wherein the horseshoe crab is selected from, and, and wherein the horseshoe crab Factor C protein is produced through being recombinantly expressed from a Chinese Hamster Ovary (CHO) DG44 cell or HEK cell.

Recombinant Factor C and method for producing the same, and method for measuring endotoxin

To provide a method for producing a horseshoe crab recombinant Factor C. The horseshoe crab recombinant Factor C is produced through expression thereof by use of mammalian cells such as CHO DG44 and HEK293 as host cells.

Recombinant factor C and method for producing the same, and method for measuring endotoxin

The present invention relates to polypeptide having a primary structure of amino acid sequence shown by Sequence List Sequence No. 1 and DNA encoding for the polypeptide. The polypeptide is obtainable by following steps (1)-(3):

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Fukuoka, Japan

Shun-ichiro Kawabata