Canon U.S. Life Sciences, Inc.

Canon U.s.a., Inc.

Theranos, Inc.

Other

Labrador Diagnostics LLC

Shulin Zeng

Kenton C Hasson

Gregory A Dale

Brian P Murphy

Hiroshi Inoue

Gregory H Owen

Ray Tsao

Timothy Michael Kemp

Johnathan Stuart Coursey

John K Howard

Chris Todd

Hongye Liang

Jeff Fenton

Ron Oral

Eric D Schneider

Alex K Flamm

Ben Lane

Ivor T Knight

Scott Corey

Weidong Cao

Conrad Laskowski

Sami Kanderian

Michele R Stone

Brian Bean

Takayoshi Hanagata

Franklin Regan

Ying-Xin Wang

Dwayne W Warfield

Elizabeth A Holmes

John Patrick Keady

Ian Gibbons

Kunihiro Sakai

Shaunak Roy

George Maltezos

Chengwang Wang

Arvind Virmani

Shize Daniel Qi

Hanyoup Kim

Makoto Ogusu

Harini Shandilya

Melissa Gosse

Yoichi Murakami

Brian Scrivens

Yasuyuki Numajiri

Christopher Sneeder

Denis Alias

Sarah Warfield, Legal Representative

Abhijit Dohale

Jason Zsak

Tim Kemp

John Howard

This invention is in the field of medical devices. Specifically, the present invention provides fluidic systems having a plurality of reaction sites surrounded by optical barriers to reduce the amount of optical cross-talk between signals detected from various reaction sites. The invention also provides a method of manufacturing fluidic systems and methods of using the systems.

Fluidic medical devices and uses thereof

The present invention relates to sample-to-answer systems, devices, cartridges, and method of using the same for detecting the presence of microorganisms in a sample, such as bacteria.

Sample-to-answer system for microorganism detection featuring target enrichment, amplification and detection

An apparatus for performing a thermocyclic process, such as amplifying DNA, includes a microfluidic chip with a channel formed therein and one or more thermal distribution elements disposed over portions of the chip. Each thermal distribution element is configured to distribute thermal energy from an external thermal energy source substantially uniformly over the portion of the chip covered by the thermal distribution element. The portion of the chip covered by the thermal distribution element thereby comprises a discrete temperature zone. Other temperature zones can be defined by other thermal distribution elements or by portions of the chip not covered by a thermal distribution element. The channel is configured so that a fluid flowing through the channel would enter and exit the different temperature zones a plurality of times, thereby alternately exposing the fluid to the temperature of each zone for a period of time required for the fluid to traverse the zone.

Systems and methods for amplifying nucleic acids

The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.

System and method for serial processing of multiple nucleic acid assays

The invention relates to systems and methods including a combination of thermal generating device technologies to achieve more efficiency and accuracy in PCR temperature cycling of nucleic samples undergoing amplification.

Combined thermal devices for thermal cycling

An interface cartridge for a microfluidic chip, with microfluidic process channels and fluidic connection holes at opposed ends of the process channels, provides ancillary fluid structure, including fluid flow channels and input and/or waste wells, which mix and/or convey reaction fluids to the fluidic connection holes and into the process channels of the microfluidic chip.

Fluid interface cartridge for a microfluidic chip

A system for amplifying nucleic acids is disclosed which, in one embodiment, includes a fluidic device having a sample channel and a heat exchange channel disposed sufficiently close to the sample channel such that a heat exchange fluid in the heat exchange channel can cause a sample in the sample channel to gain or lose heat at desired levels. In one illustrative embodiment, the system further includes three reservoirs coupled to the heat exchange channel and a temperature control system configured to heat fluids stored in the respective reservoirs at different temperatures. One or more pumps and a controller are configured to cause fluid stored in the reservoirs to enter and flow through the heat exchange channel at different times.

Systems and methods for the amplification of DNA

A system and method for controlling fluid flow within a microchannel includes a fluid circuit comprising a fluid outlet well and one or more fluid inlet wells, all in communication with a microchannel. A negative pressure differential is applied to the outlet well and fluid flow from an inlet well into the microchannel is controlled by opening or closing the inlet well to atmospheric pressure. To stop fluid flow from the inlet well, a negative pressure differential may be applied to the inlet well to equalize pressure between the inlet and outlet wells. By sequentially opening and closing different inlet wells to atmosphere, controlled amounts of different reagents can be serially introduced into the microchannel.

System and method for microfluidic flow control

In one aspect, the present invention provides a systems and methods for the real-time amplification and analysis of a sample of DNA.

Systems and methods for real-time PCR

Growing community of inventors

Gaithersburg, MD, United States of America

Shulin Zeng