Anticancer, Inc.

Shukuan Li

Yuying Tan

Zhijian Yang

Xinghua Sun

Shigeo Yagi

Qinghong Han

Mingxu Xu

Robert M Hoffman

Sant Chawla

Kei Kawaguchi

A composition and method for lowing serum and plasma levels of methionine by oral administration. The composition includes a recombinant methioninase enzyme and a cofactor (pyridoxal-L-phosphate). Methods of use describe methods for treatment of cancer, including malignant melanoma, by oral administration of the methioninase composition. Methods for chronic suppressive therapy of melanoma and other cancers are described. Because reduction of plasma methionine levels is effective in treating other conditions, including diabetes and conditions associated with aging, the use of the methods described herein includes treatment of these and other conditions.

Orally administered composition to lower serum methionine levels and method of use

A composition, method and kit for performing a two-reagent enzymatic homocysteine assay, wherein a single homocysteinase enzyme and a Schiff-based conjugate of N,N-dibutyl-p-phenyldiamine (DBPDA) with pyridoxal 5'-phosphate (PLP) are used to measure total homocysteine in plasma or serum. The assay measures a chromophore reaction product of HS and the DBPDA released from the Schiff-base conjugate in the presence of a Fecontaining compound. The resulting chromophore may be measured absorbance or fluorescence spectrophotometry.

Schiff-base conjugate of N, N-dibutyl-P-phenylenediamine with pyridoxal 5'-phosphate for improved homocysteine assays using pyridoxal 5&#x2032;-phosphate-dependent enzymes

The present invention relates to highly conjugated proteins and methods for making such proteins. In particular, the present invention relates to methods for linking additional sites to a protein for conjugation with activated polyethylene glycol (PEG) linkers, without denaturing the protein. The invention also relates to highly conjugated proteins with decreased immunogenicity and increased circulating half-life.

Methods for increasing protein polyethylene glycol (PEG) conjugation

Infection bycan be treated by administering methioninase optionally in combination with additional therapeutic agents, such as antibiotics.

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San Diego, CA, United States of America

Shukuan Li