Mgi Tech Co., Ltd.

Bgi-shenzhen Co., Limited

Shujin Fu

Hui Fu Jiang

Wenwei Zhang

Chunyu Geng

Lingyu He

Radoje Drmanac

Yuan Jiang

Dennis G Ballinger

Xia Zhao

Andrei Alexeev

Yanyan Zhang

Yaqiao Li

Xiaoshan Su

Fanzi Wu

Hui Jiang

Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic acid strand, the 5' end of the long strand has a phosphoric acid modification, and the 3&#x2032; end of the short strand has an enclosed modification, with enzyme sites in the short strand; and the linker B is a nucleic acid single strand, and the 3&#x2032; end thereof can be in a complementary pairing with the 5&#x2032; end of the long strand of the linker A. Using the linker element of the present invention for constructing a sequencing library ensures the linking directionality of the linkers while solving the problems of fragment interlinking, linker self-linking and low linking efficiency, and reducing the purification reaction between steps, shortening the linking time and reducing costs.

Linker element and method of using same to construct sequencing library

Provided are an isolated oligonucleotide and a use thereof in nucleic acid sequencing, wherein the isolated oligonucleotide comprises a first strand, wherein the 5'-end nucleotide of the first strand has a phosphate group, and the 3&#x2032;-end nucleotide of the first strand is a dideoxynucleotide, and a second strand, wherein the 5&#x2032;-end nucleotide of the second strand does not have a phosphate group, and the 3&#x2032;-end nucleotide of the second strand is a dideoxynucleotide, wherein the first strand is longer than the second strand in length, and a double-stranded structure is formed between the first strand and the second strand.

Isolated oligonucleotide and use thereof in nucleic acid sequencing

Growing community of inventors

Shenzhen, China

Shujin Fu