Wako Pure Chemical Industries, Inc.

Shuji Matuura

Masakazu Tsuchiya

Kenji Nakamura

Shinji Satomura

Masaaki Kida

Kazunisa Kubotsu

Two or more analytes having the same action, or having different actions in spite of their similar structures, or two or more analytes having the same action and the same detectable chemical characteristics, in samples derived from living bodies, etc., can be measured rapidly and easily by forming one or more complexes with one or more affinity substances, separating the complexes by using high pressure liquid chromatography, followed by measurement of the amount of an affinity substance or one of the analytes.

Process for separating and measuring trace components

Measuring of endotoxin using a reaction of a horseshoe crab hemocyte lysate with endotoxin in a solution can be carried out in the presence of a water-soluble polysaccharide containing .beta.-1,3-glucosidic linkage and/or a water-soluble polysaccharide derivative containing .beta.-1,3-glucosidic linkage.

Process for measuring endotoxin

A combination of reagents including (i) a first liquid containing (a) first microcapsules having an analyte immobilized on surfaces thereof and containing a marker therein and (b) microcapsules encapsulating an antibody and having different capsule walls from said first analyte immobilized microcapsules with respect to susceptibility to a capsule wall lysin, and (ii) a second liquid containing complement is suitable for immunoassay based on complement-dependent immune lysis of microcapsules.

Immunoassay process and liquid reagents used therefor

A reagent obtained by bringing an extracted solution of horseshoe crab hemocyte lysate into temporary contact with a treating agent such as a water-insoluble polysaccharide containing .beta.-1,3-glucosidic linkage and freeing the extracted solution from said treating agent is effective for specifically measuring endotoxin.

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Kawanishi, Japan

Shuji Matuura