Asahi Glass Company, Limited

Jmtc Enzyme Corporation

Agc Inc.

Shuichiro Kimura

Futoshi Hara

Takayuki Tanaka

Hideki Tohda

Yu Hanawa

Kenji Kitaoka

Mayumi Kobayashi

Yuichiro Hagiya

Shuji Yamazaki

Susumu Zen-In

Ayako Kashima

The present invention relates to a transformant which usesas a host into which a D-LDH gene derived from bacteria of the genusand a D-LDH gene derived from bacteria of the genusare incorporated and in which some of the genes in a group of pyruvate decarboxylase-encoding genes of thehost have been deleted or inactivated.

Transformant and process for production thereof, and process for production of lactic acid

There is provided a glass substrate for observing minute substance, made of porous glass and capable of separating and capturing a minute substance with a 10 to 500 nm average particle diameter contained in a solution or a suspension, comprising a porous glass substrate having a plurality of pores, wherein the plurality of pores has an average pore diameter ranging from 30 to 110% of the average particle diameter of the minute substance, each of the plurality of pores has a surface pore diameter on an uppermost surface of the glass substrate, a standard deviation of the surface pore diameter is 60% or less of the average particle diameter of the minute substance, and a pore with a pore diameter ranging from 60 to 140% of a pore diameter at peak top in a pore diameter distribution of the plurality of pores occupies 90% or more of total pore volume.

Device and method for observing and filter for capturing a minute substance

Provided is a transformant which can produce lactic acid with a high productivity without requiring neutralization with an alkali and is excellent in both of lactic acid production capability and growth ability and its production process, and a method for producing lactic acid by using the transformant. A transformant comprising 3 copies of a human lactate dehydrogenase gene that is introduced into ahost, wherein a gene encoding pyruvate decarboxylase 2 of thehost is deleted or inactivated.

Transformant and its production process, and method for producing lactic acid

Provided is a transformant ofmutant which can produce and collect &#x3b2;-glucosidase without requiring complicated separation steps, and a vector which is useful for transforming a yeast of the genus. The transformant of amutant of the present invention is a transformant of amutant which exhibits an increased Gsf activity and decreased or no pyruvyl transferase Pvg1 enzymatic activity, and is comprised of a structural gene sequence encoding a &#x3b2;-glucosidase derived from a filamentous fungus, and a promoter sequence and a terminator sequence for expressing the structural gene in a chromosome or as an extrachromosomal gene. Further, the present invention relates to a cloning vector which is characterized by comprising a hsp9 gene promoter or an ihc1 gene promoter of a yeast of the genus, and is useful for transforming a yeast of the genus, an expression vector, a transformant, etc.

[object Object]

Objects of the present invention are to provide a transformant which can produce lactic acid with high productivity without requiring neutralization with an alkali, a method for producing the same, and a method for producing lactic acid by using the transformant. Namely, they are a transformant, in whichis used as a host, a lactate dehydrogenase gene ofis introduced, and a part of a gene cluster encoding a pyruvate decarboxylase in thehost is deleted or inactivated; a method for producing the transformant; and a method for producing lactic acid by using the transformant.

Growing community of inventors

Tokyo, Japan

Shuichiro Kimura