Olympus Corporation

Shuhei Horigome

Kenichi Kusaka

Eiji Yokoi

Masayoshi Saito

Tadashi Watanabe

Noriyuki Sugizaki

Takuma Sukigara

An immersion microscope objective formed of thirteen or fewer lens elements includes, in order from the object side, first and second lens groups of positive refractive power, a third lens group, a fourth lens group having negative refractive power with its image-side surface being concave, and a fifth lens group having positive refractive power with its object-side surface being concave. The first lens group includes, in order from the object side, a lens component that consists of a lens element of positive refractive power (when computed as being in air) and a meniscus lens element having its concave surface on the object side. Various conditions are satisfied to ensure that images of fluorescence, obtained when the immersion microscope objective is used in a laser scanning microscope that employs multiphoton excitation to observe a specimen, are bright and of high resolution. Various laser scanning microscopes are also disclosed.

Immersion microscope objective and laser scanning microscope system using same

A construction of an objective is configured which includes a plurality of lens groups including at least one cemented lens group; a plurality of inner frames to hold the lens groups, each including a contact projection; and a tube to hold the plurality of inner frames stacked inside. At least one cemented lens group is secured to the inner frames by causing the periphery of a cemented surface between cemented lenses to come into contact with the contact projection.

Construction of objective

A microscope has a light source that emits light to illuminate a sample, a first wavelength selection member that selectively transmits the light from the light source, a light splitter that reflects the light from the first wavelength selection member to epi-illuminate the sample and transmits the light emitted from the sample, and a second wavelength selection member that selectively transmits the light transmitted through the light splitter. The light splitter is constituted of a transparent member and a dichroic mirror coat disposed on the transparent member. The dichroic mirror coat is constituted of a stacked layer that efficiently reflects light having a wavelength selected by the first wavelength selection member and a stacked layer that reflects light on a short wavelength side from the light selected by the first wavelength selection member, light on a long wavelength side, light of the same band, or combined light.

Growing community of inventors

Ina, Japan

Shuhei Horigome