Quest Diagnostics Investments, Inc.

Other

Shuguang Li

Richard E Reitz

Michael P Caulfield

W Henry Benner

Ronald M Krauss

Earl Cornell

Gloria Kwangja Lee

Patricia J Blanche

Brandon L Randall

Bingfang Yue

Wenzhe Li

Raveendra Pottathil

The invention provides methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention.

Lipoprotein analysis by differential charged-particle mobility

The present invention provides methods for determining the amount of cis-diol containing compounds in a biological samples using mass spectrometry, particularly catecholamines (dopamine, epinephrine, norepinephrine) in a plasma sample. After a sample is mixed with an internal standard, extracted with a two-step liquid-liquid extraction process utilizing phenylboronic acid chemistry, and then derivatized with a benzoyl chloride-like agent, relevant moieties are then separated chromatographically and detected by MS/MS whereby specific ion transitions are monitored. This invention has several clinical utilities, particularly those related to measuring plasma catecholamines in patients.

Sensitive method for measuring cis-diol containing compounds in plasma using 2D-LC-MS/MS

Optical bio-discs for biochemical analysis, bio-disc analysis systems and biochemical analysis methods are described herein. In one embodiment, the bio-disc includes a sample analysis circuit that includes a separation membrane for separating an investigational feature of a sample. The bio-disc also includes a conjugate release pad, and an analysis membrane containing analysis zones that may be analyzed for the presence of analytes. An analysis method includes providing a sample to a separation membrane in a bio-disc, separating an investigational feature from the sample using a separation membrane, mixing reagents that include signal elements for detecting an analyte with the investigational feature so as to form a reagent-investigational feature mixture and capturing an analyte with a signal element bound thereto in an analysis zone on an analysis membrane. The method further includes determining the presence of an analyte by analyzing the signal elements present in the capture zone of the analysis membrane.

Methods and apparatus for blood separation and analysis using membranes on an optical bio-disc

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Rancho Santa Margarita, CA, United States of America

Shuguang Li