National Science Council

Tatung Company

Shuen-Fuh Lin

Ying-Chieh Tsai

Min-Chang Huang

Kuang-Hsiang Chuang

Chien-Ming Chiou

Yuan-Chi Su

The method to produce itaconic acid is a solid-state fermentation method. Sugarcane pressmud or peeled sugarcane pressmud is the support used to adsorb liquid medium for the production of itaconic acid by an,mutant strain. This mutant strain was derived from,ATCC 10020 by successive mutation. Beside the remaining sucrose in the sugarcane pressmud, other carbon source can be added, i.e. glucose, fructose, sucrose, or starch hydrolysate. Appropriate amounts of nitrogen source, such as ammonium nitrate, or ammonium sulfate are added. Mineral salts, such as potassium dihydrogen phosphate, magnesium sulfate, calcium sulfate, ferric chloride, zinc sulfate, and copper sulfate can be added to the medium. The suitable amount of liquid medium that can be added to the support is 4 to 6 times its dry weight for the sugarcane pressmud and 8 to 14 times its dry weight for the peeled sugarcane pressmud. The optimal pH of the medium is between 2.0-3.0. The fermentation temperature is between 30-40 degrees centigrade.

Method for the production of itaconic acid using aspergillus terreus solid state fermentation

A DNA fragment obtained from Pseudomonas pseudoalcaligenes F-111, the DNA fragment comprises a base sequence of encoding alkaline lipase gene and a base sequence of controlling the expression of encoding alkaline lipase gene. This invention also discloses the process of using these DNA fragments.

Cloning, expression and nucleotide sequence of an alkaline lipase gene

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Shuen-Fuh Lin