The Johns Hopkins University

University System of Maryland

Shuang Yang

Hui Zhang

Shuwei Li

Highly specific and novel methods for analyzing glycans and proteoglycans are provided. The present invention provides glycan-reactive isobaric Aldehyde-Reactive Tags (iARTs) and the quantification of iARTs-labeled glycans by tandem mass spectrometry. The iARTs have an amine as an active group to react with aldehyde at the reducing end of glycans through reductive amination and demonstrated complete labeling. Due to the isobaric nature of the iARTs, differentially labeled glycans do not differ in mass, and quantitative information is provided by the isotope-encoded reporter ions generated from MS/MS or MSspectra. Quantitative information is thus derived from signal of the reporter ions on same precursor. Uses of the information generated by the inventive methods for diagnosis and treatment are also disclosed.

Isobaric aldehyde-reactive tags for and analysis of glycans using same

The disclosure provides quaternary ammonium containing isobaric tag reagents useful in the analysis of biomolecules and methods of making and using the quaternary ammonium containing isobaric tag reagents. The quaternary ammonium containing isobaric tag reagents are particularly useful for glycan analysis, especially quantitative glycan profiling such as glycan quantitation by tandem mass spectrometry.

Quaternary ammonium containing isobaric tag for quantitative glycan profiling

Highly specific and novel methods for analyzing glycans and proteoglycans are provided. Uses of the information generated by the inventive methods for diagnosis and treatment are also disclosed.

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Ellicott City, MD, United States of America

Shuang Yang