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Shuang Li

He Huang

Qing Xu

Zhen Gao

Astrain, mutagenesis and screening methods thereof, and methods of producing fumaric acid by fermentation. The strain is named asME-F13, and deposited in China Center for Type Culture Collection with depository number CCTCC M 2010351. The strain is obtained by physically mutagenizing the original strain ME-F12 through ion injection, culturing the processed bacteria on the solid selective plate containing 2-D-deoxylucose (2-DG) and picking up 2-DG-resistant single colony. The strain is capable of simultaneously saccharifying starchy material and fermenting it to produce fumaric acid. With an improved enzymatic activity, the strain can be directly used to ferment raw starchy materials without needing pre-saccharifying.

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Jiangsu, China

Shuang Li