Bristol-myers Squibb Compnay

Shu-Jen Chiang

Li You

Thomas J Franceschini

Jonathan D Basch

Gregory L Stein

Akbar Nayeem

Paul M Skonezny

Zizhuo Xing

Wendy L Anderson

Nigel Ian Bowers

Yuhua Sun

Suo-Win Liu

Brian L Davis

Daniel A Benigni

William V Burnett

Suo Win Liu

Sean M Tonzi

Gabriel M Galvin

Thomas P Tully

Dennis Gu

David Hou

Les Mintzmyer

Bruce Eagan

Ivan Hargro

Mark Mascari

Cary W McConlogue

Fahri T Comezoglu

Hsing Hou

Robert Stankavage

Bi-cistronic plasmids used for the expression of formate dehydrogenase (FDH) and modified phenylalanine dehydrogenase (PDHmod) are provided.

Genetically stable plasmid expressing PDH and FDH enzymes

The present invention relates to a process for preparing (2R,3S)-1-halo-2-hydroxy-3-(protected)amino-4-substituted butane comprising contacting a mutagenizedhaving ATCC deposit no. PTA-6648 with (3S)-1-halo-2-oxo-3-(protected)amino-4-substituted butane substrate. The present invention also relates to a process comprising mixing (2R,3S)-1-halo-2-hydroxy-3-(protected)amino-4-substituted butane with at least one base in the presence of at least one solvent to produce a reaction mixture containing (2R,3S)-1,2-epoxy-3-(protected)amino-4-substituted butane. The present invention further relates to a process comprising crystallizing the (2R,3S)-1,2-epoxy-3-(protected)amino-4-substituted butane out of the reaction mixture by concurrently adding water and the reaction mixture together. The present invention is also directed to a mutagenizedhaving ATCC deposit no. PTA-6648.

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Process for preparing (2R,3S)-1,2-epoxy-3-(protected)amino-4-substituted butane and intermediates thereof

Isolated nucleic acid sequences and polypeptides encoded thereby for epothilone B hydroxylase and mutants and variants thereof and a ferredoxin located downstream from the epothilone B hydroxylase gene are provided. Also provided are vectors and cells containing these vectors. In addition, methods for producing recombinant microorganisms, methods for using these recombinant microorganism to produce hydroxyalkyl-bearing epothilones and an epothilone analog produced by a mutant of epothilone B hydroxylase are provided.

Compositions and methods for hydroxylating epothilones

The present invention relates to improved methods for the production, isolation and purification of epothilone B. These methods include, for example, a fermentation process for the production of epothilone B, isolation via adsorption onto a resin, and subsequent purification.

Methods for the preparation, isolation and purification of epothilone B, and x-ray crystal structures of epothilone B

Nucleic acids coding for penicillin V amidohydrolase (PVA) from Fusarium oxysporum. Also, expression vectors, host cells and a method for production of PVA.

Penicillin V amidohydrolase gene from Fusarium oxysporum

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Manlius, NY, United States of America

Shu-Jen Chiang