Grape King Bio Ltd

Shu-Hsing Yeh

Chin-Chu Chen

Li-Ya Lee

Jui-Hsia Hsu

Han-Hsin Chang

Pei-Cheng Lin

Yen-Lien Chen

Lee-Sar Sheng

A method of preparingmycelium active substances for preventing and/or treating xerophthalmia is provided. The method comprises: (a) culturing amycelium in a plate media at 15 to 35&#xb0; C. for 5 to 14 days; (b) inoculating the mycelium of step (a) to a flask containing liquid media and culturing it at 15 to 35&#xb0; C. with a pH of 2 to 8 for 3 days; (c) inoculating the mycelium of step (b) to a fermenter tank and culturing it at 15 to 35&#xb0; C. with a pH of 2 to 8 for 3 days, so as to obtain amycelium fermentation liquid; (d) freeze-drying and grating the fermentation liquid, so as to obtain amycelium powder; (e) extracting the powder with solvent, so as to obtain amycelium extract; and (f) drying the extract, so as to obtained themycelium active substances.

mycelium active substances, preparation method, pharmaceutical composition and application thereof

The present invention provides a method for preventing, postponing or treating ocular diseases, including changes in the anterior/posterior chamber volume, vitreous humour, and/or retinal detachment. The method comprises administering to a subject an effective amount ofmycelium active substances. A method for preparingmycelium active substances comprises following steps: (a) culturing amycelium on a plate medium between 15-35&#xb0; C. for 5-14 days; (b) inoculating themycelium of step (a) into a flask and culturing the mycelium between 15-35&#xb0; C. and pH 2-8 for 3-7 days; and (c) inoculating themycelium of step (b) into a fermenter tank and culturing the mycelium by stirring between 15-35&#xb0; C. and pH 2-8 for 3-5 days, so as to obtain amycelium fermentation liquid containing themycelium active substances.

Method for preventing, postponing or treating changes in the anterior/posterior chamber volume, vitreous humour, and/or retinal detachment

The present invention discloses themycelia, the active substances ofmycelia, its submerged fermentation product and its pharmaceutical composition for preventing, delaying or treating cataracts, and the methods for preparing the products above. Themycelia, the active substances ofmycelia, its submerged fermentation product and its pharmaceutical composition further can be prepared as a health food for preventing or delaying cataracts.

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Taoyuan, Taiwan

Shu-Hsing Yeh