Usv Private Limited

Shrikant Mishra

Divya Lal Saksena

Chandrakesan Muralidharan

Nikhil Umesh Mohe

Rakesh Shekhawat

Nilesh Dagdu Patil

Dipanwita Maiti

Niren Praful Thakar

Mandar Ravindra Maduskar

Anjali Chutke

Priti Thakur

Bipinchandra Rathod

Gulnaz Zaidi

Narasimha Kumar Kopparapu

Meera Sankarankutty

Uma Sankararaman

Prabhakar Milind Niphadkar

Ahmed Monsur Borbhuiya

Srinivas Laxmi Rao

Dipanwita 701 Raman Building Maiti

Deepa Mhatre

Laxmi S Rao

Monsur Ahmed Borbhuiya

The present invention discloses a process for the preparation of rhPTH (1-34) also known as teriparatide by construction of a novel nucleotide, as an NcoI.IXhoI fragment as set forth in SEQ. ID. No.:1 encoding a chimeric fusion protein as set forth in SEQ.ID. No.:2 comprising of a fusion partner consisting of 41 amino acids belonging to&#x3b2;-galactosidase (LacZ) gene, an endopeptidase cleavage site, rhPTH (1-34) gene fragment, cloning the said nucleotide in an expression vector under the control of T7 promoter, transformingwith the said vector and expressing the chimeric fusion protein in fed batch fermentation. The present invention further discloses a low feed rate lactose induction for optimized expression of rhPTH (1-34) in. The present invention also discloses an unique, novel two step orthogonal purification process for rhPTH (1-34) comprising of cation exchange chromatography optionally followed by preparative chromatography selected from HIC or RP-HPLC to yield a target protein of &#x2267;99% purity. The present invention discloses a simple, cost-effective, environmentally benign method of producing high purity rhPTH (1-34).

Orthogonal process for purification of recombinant human parathyroid hormone (rhPTH) (1-34)

The present invention relates to an improved process for the preparation of N-(aminoiminomethyl)-N-(3-mercapto-1-oxopropyl)-L-lysylglycyl-L-&#x3b1;-aspartyl-L-tryptophyl-L-prolyl-L-cysteinamide, cyclic(1&#x2192;6)-disulfide of formula (1), which involves assembling a peptide chain comprising of six amino acids and a thioalkyl carboxylic acid in a required sequence on a solid support to obtain a peptide bound resin of formula (2), capping the free amino groups after each coupling, cleaving Dde group in the peptide of formula (2) from the solid support to obtain peptide-solid support of formula (3), guanylating the peptide of formula (3) at &#x3b5;-lysine-NHin an organic solvent to obtain peptide-solid support of formula (4), cleaving and deprotecting all groups in the peptide of formula (4) from the solid support to obtain peptide-amide formula (5), oxidizing the SH-peptide of formula (5) with an appropriate oxidizing agent to obtain the crude peptide-amide of formula (1) and purifying the crude peptide-amide of formula (1) by chromatographic technique. The solid support is either resin or a cellulose support like cotton, gauze, fabric, paper and perloza beads. The described process is simple, easy, environment friendly, takes lesser time and more cost effective.

Solid phase Fmoc chemistry process to prepare peptides

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Mumbai, India

Shrikant Mishra