University of Florida Research Foundation, Incorporated

Other

Shouguang Jin

Robert William Huigens, Iii

Marie B Chow

Konstantin Severinov

Candace Bichsel

Scott M Lohrke

Unhwan Ha

The present invention provides novel phenazine derivatives, such as compounds of Formula (I) and (II), and pharmaceutically acceptable salts thereof. The compounds of the invention are expected to be antimicrobial agents and may act by a microbial warfare strategy (e.g., a reactive oxygen species (ROS)-based competition strategy). The present invention also provides pharmaceutical compositions, kits, uses, and methods that involve the compounds of the invention and may be useful in preventing or treating a microbial infection (e.g., a bacterial infection) in a subject, inhibiting the growth and/or reproduction of a microorganism (e.g., a bacterium), killing a microorganism (e.g., a bacterium), inhibiting the formation and/or growth of a biofilm, or reducing or clearing a biofilm. 

Substituted phenazines as antimicrobial agents

A modifiedtype III secretion system has been developed that efficiently delivers selected proteins into a host cell. In one example, a functional nuclear Cre Recombinase is injected into embryonic stem (ES) cells and can be used to induce pluripotent stem (iPS) cells. This method of in vitro lineage directed differentiation prevents insertional mutagenesis and provides a route to selected stem cell renewal and cell-based therapies.

Bacterial mediated delivery of nuclear protein into pluripotent and differentiated cells

Nucleic acids and polypeptides involved in regulation of membrane permeability in bacteria are disclosed. Also disclosed are methods of increasing sensitivity to antibiotics in multi-drug resistant bacteria by increasing expression of PprA or PprB proteins in bacterial cells, and methods for identifying compounds that modulate PprA/PprB expression.

Modulation of bacterial membrane permeability

The present invention is directed to a method for expression of at least one heterologous gene in a host cell comprising transforming a host cell with at least one nucleic acid construct comprising a complete &agr; subunit of an RNA polymerase or a portion thereof of a hybrid nucleic acid containing a portion of the &agr; subunit of an RNA polymerase obtained from the same genus as the heterologous gene, and transforming the host cell, with at least one heterologous gene; and culturing the transformed host cell. The present invention further is directed to nucleic acid molecules used in the present method, vectors containing these nucleic acid molecules, and host cells containing the nucleic acid molecules. The nucleic acid encoding the &agr; subunit of an Agrobacterium RNA polymerase and the corresponding amino acid sequence and portions thereof is disclosed.

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Gainesville, FL, United States of America

Shouguang Jin