Nikon Corporation

Shota Tsuchida

Satoshi Ikeda

Kei Horie

A quantitative phase image generating method for a microscope, includes: irradiating an object with illumination light; disposing a focal point of an objective lens at each of a plurality of positions that are mutually separated by gaps &#x394;z along an optical axis of the objective lens, and detecting light from the object; generating sets of light intensity distribution data corresponding to each of the plurality of positions based upon the detected light; and generating a quantitative phase image based upon the light intensity distribution data; wherein the gap &#x394;z is set based upon setting information of the microscope.

Quantitative phase image generating method, quantitative phase image generating device, and program

It is aimed to prevent excitation light from leaking and being detected by the detector in a fluorescence microscope. A microscope includes an illumination optical system configured to illuminate a specimen with excitation light, a detector configured to detect fluorescence emitted from the specimen, and an observation optical system configured to guide the fluorescence to the detector. The observation optical system includes an optical filter having a variable wavelength characteristic, a light dispersing element configured to disperse, into light beams, light that has passed through the optical filter, and a light block plate configured to block at least some of the light beams resulting from the dispersion by the light dispersing element.

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Tokyo, Japan

Shota Tsuchida