Chugai Seiyaku Kabushiki Kaisha

Dai-ichi Seiko Co., Ltd.

Shota Tanaka

Takayoshi Endo

Naoki Okamoto

Takuya Takeda

Yoshiyuki Ono

Atsushi Ohta

Jun Mukunoki

Tetsuo Kojima

Atsushi Kimbara

Hirotaka Kashiwagi

Kenichi Nomura

Eisaku Mizuguchi

Nobuyuki Hori

Kazutaka Tachibana

Yoshihito Ohtake

Yoshihisa Murata

Takeo Harada

Mitsuaki Ide

Yasuhiro Ichida

Shuichi Ohtomo

Naoshi Horiba

An objective of the present invention is to provide methods of synthesizing peptides containing structurally diverse amino acids using cell-free translation systems, which can accomplish excellent translational efficiency as compared to conventional techniques (the conventional techniques being methods which involve preparing aminoacyl-tRNAs which do not have protecting groups outside the translation systems without using ARS, and then adding the prepared aminoacyl-tRNAs into translation systems). In the present invention, it was found that amino acid-containing peptides can be synthesized efficiently by protecting an amino acid linked to tRNA with an appropriate protecting group, and then performing the step of deprotecting the protecting group of the amino acid linked to tRNA and the step of peptide translation from a template nucleic acid in a cell-free translation system in parallel.

Method for synthesizing peptides in cell-free translation system

The present invention provides a dihydropyridazine-3,5-dione derivative or a salt thereof, or a solvate of the compound or the salt, a pharmaceutical drug, a pharmaceutical composition, a sodium-dependent phosphate transporter inhibitor, and a preventive and/or therapeutic agent for hyperphosphatemia, secondary hyperparathyroidism, chronic renal failure, chronic kidney disease, and arteriosclerosis associated with vascular calcification comprising the compound as an active ingredient, and a method for prevention and/or treatment.

Dihydropyridazine-3,5-dione derivative and pharmaceuticals containing the same

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Shizuoka, Japan

Shota Tanaka