University of California

Shota Atsumi

James Liao

Gabriel M Rodriguez

Anthony F Cann

Kevin M Smith

Roa Pu Claire Shen

Yohei Tashiro

Michael R Connor

Justin Bloomfield Siegel

Michael D Toney

Shuchi H Desai

John W K Oliver

Iara M P Machado

D Alexander Carlin

Irina Koryakina

The present disclosure provides recombinant bacteria with elevated production of ethanol and/or n-butanol from ethylene. Methods for the production of the recombinant bacteria, as well as for use thereof for production of ethanol and/or n-butanol are also provided.

Bacteria engineered for conversion of ethylene to ethanol

The present disclosure provides recombinant bacteria with elevated 2-keto acid decarboxylase and alcohol transferase activities. Some recombinant bacteria further have elevated aldehyde dehydrogenase activity. Some recombinant bacteria further have reduced alcohol dehydrogenase and/or aldehyde reductase activity. Methods for the production of the recombinant bacteria, as well as for use thereof for production of various esters are also provided.

Bacteria engineered for ester production

The present disclosure provides recombinantand other bacteria with reduced alcohol dehydrogenase and/or aldehyde reductase activity. The present disclosure further provides recombinantand other bacteria with reduced isobutyraldehyde reductase activity. Methods for the production and the uses of the recombinant bacteria are also provided. Specifically, recombinant bacteria further expressing 2-keto-acid decarboxylase can be used for producing higher aldehydes or other non-alcohol chemicals derived from aldehydes.

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Provided herein are metabolically-modified microorganisms useful for producing biofuels. More specifically, provided herein are methods of producing high alcohols including isobutanol, 1-butanol, 1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol from a suitable substrate.

Biofuel production by recombinant microorganisms

The present disclosure provides recombinant cyanobacteria having acetolactate synthase activity and acetolactate decarboxylase activity, as well as recombinant cyanobacteria having acetolactate synthase activity, acetolactate decarboxylase activity, and secondary alcohol dehydrogenase activity. Methods for the production of the recombinant cyanobacteria, as well as methods for use thereof for production of acetoin and 2,3-butanediol from carbon dioxide and light are also provided.

Methods of producing acetoin and 2,3-butanediol using photosynthetic microorganisms

Provided herein are metabolically-modified microorganisms useful for producing biofuels. More specifically, provided herein are methods of producing high alcohols including isobutanol, 1-butanol, 1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and 2-phenylethanol from a suitable substrate and a recombinant acetolactate synthase having both synthase and decarboxylase activity.

Acetolactate synthase having 2-ketoisovalerate decarboxylase activity and uses thereof

The invention provides recombinant microorganisms capable of producing isobutyraldehyde using COas a carbon source. The invention further provides methods of preparing and using such microorganisms to produce isobutyraldehyde.

Growing community of inventors

Davis, CA, United States of America

Shota Atsumi